Axin1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:147293 Chia IV, et al., Both the RGS Domain and the Six C-Terminal Amino Acids of Mouse Axin Are Required for Normal Embryogenesis. Genetics. 2009 Apr;181(4):1359-68
Assay type:	Western blot
MGI Accession ID:	MGI:3840878
Gene symbol:	Axin1
Gene name:	axin 1

Antibody:

anti-Axin

Results

Image: 1E

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	130 kDa (b)	110 kDa (c)	Genetic Background	Mutant Allele(s)	Sex
+/+	E10.5 (a)	TS17: embryo	Absent	Present	Not Specified		Not Specified
Ax/Ax	E10.5 (a)	TS17: embryo	Present (d)	Absent	involves: 129S6/SvEvTac * FVB/N	Axin1^{tm1(Axin2)Cos}/Axin1^{tm1(Axin2)Cos}	Not Specified
Ax/+	E10.5 (a)	TS17: embryo	Present (e)	Present	involves: 129S6/SvEvTac * FVB/N	Axin1^tm2Cos/Axin1⁺	Not Specified
deltaC6/+	E10.5 (a)	TS17: embryo	Weak (f)	Present	involves: 129S6/SvEvTac * FVB/N	Axin1^tm4Cos/Axin1⁺	Not Specified
deltaC6/+	E10.5 (a)	TS17: embryo	Weak (f)	Present	involves: 129S6/SvEvTac * FVB/N	Axin1^tm4Cos/Axin1⁺	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Upper bands represent Myc-tagged products.
(c) Lower band is the endogenous gene product.
(d) Myc-tagged Ax allele product.
(e) The heterozygote expressed similar amounts of Myc-tagged Ax allele product as endogenous protein from the normal allele.
(f) The Myc-tagged deltaC6 product was expressed at approximately 3 to 4-fold lower levels than the endogenous protein from the normal allele.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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