Antibody:	anti-TGFbeta3
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to biotin

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Strong	Regionally restricted		Strong expression in chondrocytes in the ribs and vertebra.
TS20: vertebral cartilage condensation	Strong	Regionally restricted		Strong expression in chondrocytes in the ribs and vertebra.
TS20: skeleton	Present	Regionally restricted		Little expression in the cartilage rudiments for other bones.

Structure	Level	Pattern	Image	Note
TS21: skeleton	Present	Regionally restricted		High expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: skeleton	Present	Regionally restricted		High expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: skeleton	Present	Regionally restricted		High expression in chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: skeleton	Present	Regionally restricted		Little expression in the periosteum. Expression in cytoplasm of osteocytes of bone and maturing chondrocytes such as those of tracheal cartilage.

Structure	Level	Pattern	Image	Note
TS25: skeleton	Present	Regionally restricted		Low levels of expression in osteogenic bone and hypertrophic cartilage.
TS25: tooth epithelium	Weak	Regionally restricted		Low levels of expression in pulp cells and ameloblasts.
TS25: tooth mesenchyme	Weak	Regionally restricted		Low levels of expression in pulp cells and ameloblasts.
TS25: tooth	Present	Regionally restricted		No expression in the odontoblast layer.

Structure	Level	Pattern	Image	Note
TS20: heart atrium	Strong		4A
TS20: heart ventricle	Strong		4A

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		4B
TS20: atrioventricular endocardial cushion	Weak		4B

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Regionally restricted	4C	Expression in an umbilical vessel.

Structure	Level	Pattern	Image	Note
TS20: blood vessel	Present	Regionally restricted	4D

Structure	Level	Pattern	Image	Note
TS21: heart	Present

Structure	Level	Pattern	Image	Note
TS22: heart	Present

Structure	Level	Pattern	Image	Note
TS23: heart	Present

Structure	Level	Pattern	Image	Note
TS24: heart	Present

Structure	Level	Pattern	Image	Note
TS25: heart	Trace			Expression barely detectable above background.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS21: renal tubule	Present	Regionally restricted		Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present	Regionally restricted		Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.

Structure	Level	Pattern	Image	Note
TS23: renal tubule	Present	Regionally restricted		Expression in the tubule epithelium.

Structure	Level	Pattern	Image	Note
TS25: renal tubule	Present	Regionally restricted		Decreased expression in the tubular epithelium.
TS25: mesenchyme of metanephros	Present	Regionally restricted		Expression in mesenchymal cells supporting the tubules.

Structure	Level	Pattern	Image	Note
TS21: gland	Weak	Regionally restricted		Only slight expression in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Absent			Authors state that expression was not present in the adrenal gland during these stages of development.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Absent			Authors state that expression was not present in the adrenal gland during these stages of development.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Absent			Authors state that expression was not present in the adrenal gland during these stages of development.

Structure	Level	Pattern	Image	Note
TS25: adrenal gland	Absent			Authors state that expression was not present in the adrenal gland during these stages of development.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Weak			Authors report slight expression in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Strong			Significantly stronger expression in the intestinal epithelium.

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present			Decreased expression compared with earlier stages.
TS25: intestine	Moderate	Regionally restricted		Moderate expression in the smooth muscle of the intestine.

Structure	Level	Pattern	Image	Note
TS25: esophagus	Present	Regionally restricted		Moderate expression in the smooth muscle of the esophagus; no expression in the stratified squamous epithelium.

Structure	Level	Pattern	Image	Note
TS25: stomach	Absent			No expression in the stratified squamous epithelium of the forestomach.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted		Expression in megakaryocytes.

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	7D	Expression in the liver capsule; no expression in hepatocytes.
TS21: liver parenchyma	Weak		7D	Low levels of expression in mesenchymal cells supporting the liver parenchyma.

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Strong		8A
TS25: lung vascular element	Strong		8A	Strong expression in large pulmonary blood vessels.

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Present	Regionally restricted	8B	Expression in the subepithelial region of the bronchioles.
TS25: lung vascular element	Present	Regionally restricted	8B	Expression in the smooth muscle cells of blood vessels.

Structure	Level	Pattern	Image	Note
TS21: inner ear	Present	Regionally restricted	9D	Expression throughout the cochlear epithelium, with highest levels in the apical region.

Structure	Level	Pattern	Image	Note
TS25: cochlea	Absent			No expression in the cochlear epithelium.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	10H	Expression in astrocytes in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present	Regionally restricted		Expression in spinal cord astrocytes.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted		Expression in spinal cord astrocytes.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Strong			Intense expression in the epithelial layer of the skin over the entire body.

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present
TS25: hair follicle	Present