Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143211
Kwakowsky A, et al., GABA neurotransmitter signaling in the developing mouse lens: dynamic regulation of components and functionality. Dev Dyn. 2008 Dec;237(12):3830-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3837348
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Gene symbol:
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Slc6a1
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Gene name:
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solute carrier family 6 (neurotransmitter transporter, GABA), member 1
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Antibody:
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anti-GAT1
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
3D:
embryonic day 11.5
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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either: C57BL/6 or FVB.129P2-Pde6b+ Tyrc-ch/AntJ
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lens vesicle |
Present |
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3D
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Expression is in the lens epithelial cells comprising the lens vesicle and is enriched at the luminal (apical) ends.
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Specimen
3E:
embryonic day 16.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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either: C57BL/6 or FVB.129P2-Pde6b+ Tyrc-ch/AntJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lens epithelium |
Strong |
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3E
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| TS24: lens fiber |
Strong |
Regionally restricted |
3E
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Expression is at the tips of secondary fiber cells of the equatorial region and in primary fibers forming the lens nucleus.
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Specimen
3F:
postnatal day 0
(more )
Specimen
3F:
(close )
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Genetic Background:
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either: C57BL/6 or FVB.129P2-Pde6b+ Tyrc-ch/AntJ
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4C:
embryonic day 16.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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either: C57BL/6 or FVB.129P2-Pde6b+ Tyrc-ch/AntJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lens epithelium |
Present |
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4C
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Expression is in the apical/basal membranes of lens epithelial cells.
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| TS24: lens fiber |
Present |
Regionally restricted |
4C
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Expression is in the apical ends of equatorial fibers and their basal tips forming the posterior lens suture and in the lateral secondary fiber membranes.
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Specimen
4D:
embryonic day 16.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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either: C57BL/6 or FVB.129P2-Pde6b+ Tyrc-ch/AntJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lens |
Present |
Regionally restricted |
4D
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Expression is in the posterior lens pole with the forming suture.
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