Gene Expression Data
Assay Details
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Assay
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Reference:
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J:15273
Ohlsson H, et al., IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J. 1993 Nov;12(11):4251-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3836601
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Gene symbol:
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Pdx1
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Gene name:
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pancreatic and duodenal homeobox 1
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Antibody:
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anti-IPF1
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Detection system:
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horseradish peroxidase
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Results
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Specimen
5A:
postnatal adult
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B:
postnatal adult
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreatic islet core |
Present |
Regionally restricted |
5B
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Expression in majority of islet cells; localized in the center of the islets. No expression in cells expressing glucagon.
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Specimen
5C:
postnatal adult
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreatic islet core |
Present |
Regionally restricted |
5C
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Expression in majority of islet cells; localized in the center of the islets cells. No expression in cells expressing somatostatin.
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Specimen
6A,B:
embryonic day 9.5
(more )
Specimen
6A,B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: foregut-midgut junction |
Present |
Regionally restricted |
6B
,
6A
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Expression in dorsal and ventral walls of the duonenum.
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Specimen
6C:
embryonic day 9.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: foregut-midgut junction |
Present |
Regionally restricted |
6C
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Expression in dorsal and ventral walls of the duonenum, and not in lateral parts of the gut wall
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Specimen
Not shown:
embryonic day 9.0
(more )
Note: 18-20 somites.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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18-20 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Present |
Regionally restricted |
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Expression in part of duodenum that will give rise to the dorsal and ventral pancreas.
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Specimen
6D:
embryonic day 8.5
(more )
Note: 13 somites.
Specimen
6D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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13 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Present |
Regionally restricted |
6D
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Expression in a few cells in dorsal wall of the duodenum.
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Specimen
Not shown:
embryonic day 8.5
(more )
Note: 13 somites.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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13 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: foregut-midgut junction |
Present |
Regionally restricted |
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Expression in a few cells in ventral wall of the duodenum.
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Specimen
Not shown:
embryonic day 8.0
(more )
Note: 10 somites.
Specimen
Not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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10 somites.
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Specimen
7A:
embryonic day 10.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7B:
embryonic day 11.5
(more )
Specimen
7B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: pancreas primordium |
Present |
Regionally restricted |
7B
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Authors state there is a drastic decrease in the relative number of expressing cells between E10.5 and E11.5.
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Specimen
7C:
embryonic day 13.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas |
Weak |
Regionally restricted |
7C
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Very few expressing cells.
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Specimen
7D:
embryonic day 15.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
7D
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Substantial increase in relative number of expressing cells. Coexpression with insulin.
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Specimen
7E:
embryonic day 15.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
Regionally restricted |
7E
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Substantial increase in relative number of expressing cells. No co-expression with glucagon.
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