Antibody:	Anti-NeuN (clone A60; MAB377)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	1A	Expression in nuclei of most neuronal cell types in all regions of the brain, including cerebral cortex, hippocampus, thalamus, caudate/putamen and cerebellum. Expression in cytoplasm usually markedly lower than in the nucleus.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	1B	Higher magnification was shown in the inset. Intense expression in large pyramidal cells; less expression in the cytoplasm; no expression in the nucleolus.
TS28: neocortex layer	Present	Regionally restricted	1B	Higher magnification was shown in the inset. Authors report expression in all cortical layers.
TS28: cerebral hemisphere white matter	Absent		1B	Higher magnification was shown in the inset.
TS28: corpus callosum	Absent		1B	Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present		1C
TS28: spinal cord ventral horn	Present		1C
TS28: spinal cord white matter	Absent		1C

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong	Regionally restricted	2A , 2B	Intense nuclear expression in granule cells.
TS28: cerebellar cortex	Strong	Regionally restricted	2B , 2A	Authors report strong expression in Golgi type II neurons and cells in the deep nuclei.
TS28: deep cerebellar nucleus	Strong	Regionally restricted	2B , 2A	Authors report strong expression in Golgi type II neurons and cells in the deep nuclei.
TS28: cerebellum Purkinje cell layer	Absent		2B , 2A	No expression in Purkinje cells or in putative Bergmann glia.
TS28: cerebellum white matter	Absent		2B , 2A	No expression in Purkinje cells or in putative Bergmann glia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present	Single cells

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present			Authors report large numbers of immunoreactive cells in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Strong	Regionally restricted	2C	Intense nuclear expression in granule cells.
TS28: main olfactory bulb mitral cell layer	Absent		2C	No expression in mitral cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Strong		2D	Intense expression of gangion cells.
TS28: retina inner nuclear layer	Present	Regionally restricted	2D	Expression in some cells along the inner margin of the inner nuclear layer; no expression in most cells in the inner nuclear layer.
TS28: photoreceptor layer	Absent		2D	No expression in photoreceptor cells in the outer nuclear layer.
TS28: retina outer nuclear layer	Absent		2D	No expression in photoreceptor cells in the outer nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	3	Nuclear expression in large hippocampal neurons; no nuclear expression in astrocytes, or in glia within the neuronal layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Absent			Authors report no immunoreactivity in reactive glia.

Structure	Level	Pattern	Image	Note
TS15: nervous system	Present			Authors state that expression is confined to the nervous system.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	4A	Expression in cells at the lateral margins of the basal plate in areas with post-mitotic cells.
TS17: midbrain floor plate	Absent		4A	No expression in the ventricular zone and floor plate.
TS17: midbrain ventricular layer	Absent		4A	No expression in the ventricular zone and floor plate.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted		Authors state that the more cranial regions of spinal cord have greater expression in ventral than in dorsal regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4C , 4B	Greater expression in ventral than in dorsal regions; large numbers of expressing cells in ventral horns; expression in dorsal horns.
TS20: spinal cord floor plate	Absent		4C , 4B	No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: spinal cord roof plate	Absent		4C , 4B	No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: spinal cord ventricular layer	Absent		4C , 4B	No expression in ventricular zone, roof plate, floor plate or marginal zone.
TS20: dorsal root ganglion	Absent		4B , 4C

Structure	Level	Pattern	Image	Note
TS25: telencephalon	Present		4D	Expression in cells throughout the cerebral hemispheres.
TS25: cortical subplate	Strong	Regionally restricted	4D	In the neocortex, most intense expression in cells appearing as a single lamina whose position suggests these may be subplate (sublayer VIb) neurons.
TS25: cerebral cortex marginal zone	Present	Regionally restricted	4D	Some expression in marginal layer which may be Cajal-Retzius cells.
TS25: piriform cortex	Strong		4D	Authors report strong expression across all layers.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present	Regionally restricted	4E	Expression in cells along the inner margin of the external granular layer (post-mitotic cells before they migrate to the mature granular layer) as wells as cells as they migrate.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted		Expression in granular layer is more cytoplasmic than nuclear.