Tg(Scgb1a1-rtTA,tetO-IL9)1Flv
Transgene Detail
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| Symbol: |
Tg(Scgb1a1-rtTA,tetO-IL9)1Flv |
| Name: |
transgene insertion 1, Richard A Flavell |
| MGI ID: |
MGI:3836062 |
| Transgene: |
Tg(Scgb1a1-rtTA,tetO-IL9)1Flv Location: unknown
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| Alliance: |
Tg(Scgb1a1-rtTA,tetO-IL9)1Flv page
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| Transgene Type: |
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Transgenic (Inducible, Inserted expressed sequence, Transactivator) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Mutation details: The CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivator (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The second construct contains a polymeric tetracycline operator (tet-O), minimal CMV promoter, IL9 cDNA, and SV40 polyadenylation signals. Both constructs were coinjected into fertilized oocytes, and dual positive founder animals were obtained. In the presence of doxycycline (dox), rtTA is able to bind in trans to the tet-O, and the VP-16 transactivator activates LIF gene transcription. In the absence of dox, rtTA binding occurs at very low levels, and only low level gene transcription is noted.
(J:130417)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:130417 Temann UA, et al., Pulmonary overexpression of IL-9 induces Th2 cytokine expression, leading to immune pathology. J Clin Invest. 2002 Jan;109(1):29-39 |
| All: |
1 reference(s) |
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Analysis Tools
