Probe:	Af9 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		1Q
TS21: cerebral cortex ventricular layer	Present		1Q
TS21: cortical plate	Present		1Q
TS21: telencephalon mantle layer	Present		1Q
TS21: cerebral cortex	Present	Regionally restricted	1Q	Expression is in the septum.
TS21: telencephalon	Present	Regionally restricted	1Q	Expression is absent from the basal ganglia.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		1R
TS21: forebrain	Present	Regionally restricted	1R	Expression extends into the hippocampal anlage, absent in the basal ganglia.
TS21: thalamus	Present	Regionally restricted	1R	Expression is in the dorsal thalamus.
TS21: epithalamus	Very strong	Regionally restricted	1R	Expression is in the epiphysis.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present		1S
TS21: telencephalon	Present	Regionally restricted	1S	Expression is absent from the basal ganglia.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Strong	Regionally restricted	2D	Expression is in the dorsal thalamus.
TS24: cerebral cortex intermediate zone	Strong		2D
TS24: cerebral cortex subventricular zone	Strong		2D
TS24: cortical plate	Strong		2D
TS24: cerebral cortex	Strong	Regionally restricted	2D	Expression is in the septum.
TS24: forebrain	Strong	Regionally restricted	2D	Expression is in cells of the lateral migratory stream.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong		2E
TS24: cerebral cortex subventricular zone	Strong		2E
TS24: epithalamus	Strong		2E
TS24: thalamus	Strong		2E
TS24: forebrain	Present	Regionally restricted	2E	Expression extends into the hippocampus.
TS24: cortical plate	Present		2E

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		2F
TS24: thalamus	Present	Graded	2F	There is a rostral to caudal gradient.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present		2J
TS27: cingulate cortex	Present		2J
TS27: hippocampus	Present		2J
TS27: telencephalon septum	Present		2J
TS27: neocortex region	Present	Regionally restricted	2J	Expression is in the caudal cortical plate parts including limbic and visual cortical areas.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present		2K
TS27: cingulate cortex	Present		2K
TS27: hippocampus	Present		2K
TS27: telencephalon septum	Present		2K
TS27: neocortex region	Present	Regionally restricted	2K	Expression is in the caudal cortical plate parts including limbic and visual cortical areas.

Structure	Level	Pattern	Image	Note
TS27: brain subventricular zone	Present		2L
TS27: cingulate cortex	Present		2L
TS27: telencephalon septum	Present		2L
TS27: neocortex region	Present	Regionally restricted	2L	Expression is in the caudal cortical plate parts including limbic and visual cortical areas.

Structure	Level	Pattern	Image	Note
TS28: brain	Absent

Structure	Level	Pattern	Image	Note
TS26: hindbrain	Strong	Regionally restricted	3C	Expression is at the isthmus of the midbrain/hindbrain.
TS26: midbrain	Strong	Regionally restricted	3C	Expression is at the isthmus of the midbrain/hindbrain.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Strong	Regionally restricted	3D	Expression is in the neuroepithelial external germinal layer and the developing Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		5B , 5A , 5C
TS24: cortical plate	Present		5B , 5A , 5C

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		5D
TS24: cortical plate	Present		5D

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		5J , 5K , 5I
TS26: cortical plate	Present		5J , 5K , 5I

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		5L
TS26: cortical plate	Present		5L

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Present		5Q	Expression is in upper level and deep level neurons in the medial cortex.

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Present		5S	Marginal staining in the lateral cortex and subventricular zone.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		5U

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		5W

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Graded	6A	There is a lateral to medial gradient.
TS26: cerebral cortex subventricular zone	Present		6A

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Graded	6B	Expression gradient in this mutant is the same as in wild-type. There is a reduction in expression in deep layer neurons that are located next to the molecular layer.
TS26: cerebral cortex subventricular zone	Present		6B	Expression in this layer is the same as in wild-type.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Graded	6C	There is a lateral to medial gradient.
TS26: cerebral cortex subventricular zone	Present		6C

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Graded	6D	Expression gradient in this mutant is the same as in wild-type. There is a reduction in expression in deep layer neurons that are located next to the molecular layer.
TS26: cerebral cortex subventricular zone	Present		6D	Expression in this layer is the same as in wild-type.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		6E
TS26: cerebral cortex	Present	Regionally restricted	6E	Absent in the molecular layer.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present	Regionally restricted	6F	Expression in the progenitors of the subventricular zone was significantly reduced in its size, and completely lost in lateral cortical regions in this mutant.
TS26: cerebral cortex	Present	Regionally restricted	6F	The area of lack of expression in the molecular layer is enlarged in this mutant.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		6G
TS26: cerebral cortex	Present	Regionally restricted	6G	Absent in the molecular layer.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		6H	Expression in the progenitors of the subventricular zone was significantly reduced in its size, and completely lost in lateral cortical regions in this mutant.
TS26: cerebral cortex	Present	Regionally restricted	6H	The area of lack of expression in the molecular layer is enlarged in this mutant.

Structure	Level	Pattern	Image	Note
TS24: pons	Present	Regionally restricted	7H	Expression is in the pontine nuclei.
TS24: hindbrain	Present	Regionally restricted	7H	Expression is in the isthmus of the midbrain/ hindbrain boundary.
TS24: midbrain	Present	Regionally restricted	7H	Expression is in the isthmus of the midbrain/ hindbrain boundary.