Probe:	Lc3 synthase cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS18: embryo	Present		6A	Most tissues demonstrated significant exrpession.
TS18: brain ventricular layer	Present		6A	There is relatively high expression in several regions of proliferative neuropeithelium in the developing central nervous system, including cerebral venticular zones.
TS18: telencephalon ventricular layer	Present		6A	There is relatively high expression in several regions of proliferative neuropeithelium in the developing central nervous system, including cerebral venticular zones.
TS18: neural tube ventricular layer	Present		6A	There is relatively high expression in the neuroepithelium of the neural tube.
TS18: dorsal root ganglion	Present		6A
TS18: trunk sclerotome	Present		6A
TS18: 1st branchial arch mandibular component	Present		6A

Structure	Level	Pattern	Image	Note
TS18: lens vesicle	Present		6C

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	7A	Widespread expression is detected throughout the rostral caudal extent of the telencephalon.
TS22: telencephalon ventricular layer	Strong	Regionally restricted	7A	There is intense expression in the ventricular and subventricular zone, with the exception of the ventromedial wall of the caudal telencephalon.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Present		7C
TS25: telencephalon ventricular layer	Strong		7C

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong		7E	Significant expression is found in thalamic nuclei.
TS25: cerebellum	Strong	Regionally restricted	7E	There is substantial expression in the precerebellar neuroepithelium and postmitotic neurons of the deep cerebell nuclei.

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present	Regionally restricted		Expression is present in the inferior olive.
TS25: pons	Present			Expression is present in pontine nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		8A Antisense	Expression is found exclusively in Purkinje cell bodies in the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		8B Antisense	Expression is found exclusively in Purkinje cell bodies in the Purkinje cell layer.

Structure	Level	Pattern	Image	Note
TS28: spleen	Present	Regionally restricted	9_Antisense	Expression is present in the follicle region. Other staining indicates that the expressing cells are B, and not T, lymphocytes.