Hprt1<tm13(Ple54-EGFP)Ems> Targeted Allele Detail MGI Mouse (MGI:3835524)

Hprt1^{tm13(Ple54-EGFP)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm13(Ple54-EGFP)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 13, Elizabeth M Simpson
MGI ID:	MGI:3835524
Synonyms:	DCX-EGFP, Hprt1^{tm13(mEMS1927)Ems}, Hprt1^{tm13(Ple54-EGFP;mEMS1927)Ems}, Ple54
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm13(Ple54-EGFP)Ems} page

Mutation
origin

Mutant Cell Lines:	mEMS1927, mEMS1938
Germline Transmission:	Earliest citation of germline transmission: J:145689
Parent Cell Line:	mEMS1218 (ES Cell)
Strain of Origin:	(B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Project Collection:	Pleiades Promoter Project

Mutation
description

Allele Type:		Targeted (Reporter)
Mutation:		Insertion
		Mutation details: The Ple54-EGFP/NLS transgene (pEMS1200) was designed with the 3687 bp Ple54 minipromoter (DCX-C; derived from a portion of the promoter and two downstream putative regulatory elements from the human doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. Detection of EGFP is strongest in soma of cells in the neurogenic regions of the adult brain: the subgranular zone of the dentate gyrus as well as the forebrain subventricular zone (rostral migratory stream and olfactory bulb). Additionally, EGFP-positive cell bodies are detected in the neuropil of the cortex, hippocampus and brainstem. Double label immunocytochemistry shows that the EGFP-positive cells of the forebrain subventricular zone and the rostral migratory stream co-express Doublecortin. (J:144244, J:164356)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1273 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1927 has been established.

References

Original:	J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:	3 reference(s)

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