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Gene Expression Data
Assay Details
Assay
Reference: J:84947 Lee B, et al., Expression of the mouse homologue for the human GCDFP-15/PIP gene during pre- and early post-natal development. Mol Cell Endocrinol. 2003 Jul 31;205(1-2):33-41
Assay type: RT-PCR
MGI Accession ID: MGI:3834869
Gene symbol: Pip
Gene name: prolactin induced protein
Probe: mSMGP/mPIP-pA, mSMGP/mPIP-pB
Probe preparation: Double stranded labelled with P32 DNA
Visualized with: Autoradiography
Assay notes: The PCR products were electrophoresed on a 1% agarose gel, transferred to nitrocellulose, and subjected to Southern blot analysis using a full length cDNA probe.
Results Image: 6D
 Sample Information Bands Other Sample Information
Lane AgeStructure 570 bp Amount Genetic BackgroundMutant Allele(s)Sex
E18 E17.5 (a) TS25: prostate bud Absent 1 µg; total RNA CD-1 Male
1d P1 TS27: prostate bud Absent 1 µg; total RNA CD-1 Male
5d P5 TS28: prostate gland Present 1 µg; total RNA CD-1 Male
4 wk P w 4 TS28: prostate gland Present 1 µg; total RNA CD-1 Male
8 wk P w 8 TS28: prostate gland Present (b) 1 µg; total RNA CD-1 Male
8 wk P w 8 TS28: prostate gland Present (b) 1 µg; total RNA CD-1 Male
10 wk P w 10 TS28: prostate gland Absent 1 µg; total RNA CD-1 Male
10 wk P w 10 TS28: prostate gland Absent 1 µg; total RNA CD-1 Male
+ ve P w 4 TS28: submandibular gland Present 1 µg; total RNA CD-1 Male
- ve Control
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression is evident in some animals at this age, although it is substantially reduced from what was seen earlier.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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