Antibody:	Anti-Basonuclin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3

Structure	Level	Pattern	Image	Note
TS21: epidermis	Weak	Widespread		Weak, cytoplasmic expression was uniformly present in the epithelial precursors of the epidermis.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Weak		1A , 1B
TS22: hair follicle	Strong	Regionally restricted	1B , 1A	Expression becomes nuclear and abundant in epithelial cells of the developing hair follicles.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Strong	Regionally restricted	1D , 1C	Intense nuclear staining appeared in basal cells that form the placodes.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Strong	Regionally restricted	1E , 1F	Intense nuclear staining appeared in basal cells that form the placodes.

Structure	Level	Pattern	Image	Note
TS27: hair follicle	Strong	Regionally restricted	2A , 2B	Intense nuclear expression was detected in the lower segment of the follicle. In the upper segment of the folicle, expression was more faint and cytoplasmic.
TS27: hair outer root sheath	Strong		2A , 2B
TS27: epidermis	Weak	Regionally restricted	2B , 2A	Expression was faint and cytoplasmic in the basal layer of the epidermis adjacent to the follicle.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	2D , 2C	Expression was detected in the outer root sheath and the matrix layer immediately adjacent to the follicular papilla.
TS28: hair root sheath matrix	Present	Regionally restricted	2D , 2C	Expression was detected in the outer root sheath and the matrix layer immediately adjacent to the follicular papilla.
TS28: hair follicle	Present	Regionally restricted	2C , 2D	Staining was weaker and cytoplasmic in the upper segment of the follicle .

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Strong	Regionally restricted	2E , 2F	Expression was nuclear and abundant in the basal layer of the outer root sheath.
TS28: hair follicle	Present	Regionally restricted	2F , 2E	In the follicular stalk, nuclear expression was present in some suprabasal cells. Staining in the upper follicular segment was weak and cytoplasmic.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Strong	Regionally restricted	2H , 2G	Expression was nuclear and abundant in the basal layer of the outer root sheath.
TS28: hair root sheath matrix	Present	Regionally restricted	2G , 2H	Expression was nuclear in both the basal layer of the matrix and a short column of epithelial cells immediately above the papilla.

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present
TS28: hair root sheath matrix	Present	Regionally restricted		Nuclear expression was detected in the epithelial cells of the deepest part of the matrix.

Structure	Level	Pattern	Image	Note
TS27: hair follicle	Present		3A , 3B	Expression is cytoplasmic and weak in the upper segment of the hair follicle. Staining is nuclear and higher intensity in the lower follicle segment.
TS27: epidermis	Weak	Regionally restricted	3B , 3A	Expression was weak and cytoplasmic in the basal layer of the epidermis. Staining in the stratum corneum is non-specific.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	4B , 4A	Nuclear staining was detected in the outer root sheath cells surrounding the differentiating club hair.
TS28: hair follicle	Present	Regionally restricted	4B , 4A	Expression was detected in the cells of the epithelial strand connecting the presumptive club to the follicular papilla.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	4D , 4C	Nuclear staining was detected in the outer root sheath cells surrounding the differentiating club hair.
TS28: hair follicle	Present	Regionally restricted	4C , 4D	Expression was detected in the cells of the epithelial strand connecting the presumptive club to the follicular papilla.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	5A , 5B	Nuclear expression was detected in the outer root sheath encasing the club. Expression decreased in the outer root sheath cells near the upper end of the club.
TS28: hair follicle	Present	Regionally restricted	5A , 5B	Expression was detected in a peg beneath the club in close contact with the follicular papilla.

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	5D , 5C	Nuclear expression was detected in the outer root sheath encasing the club. Expression decreased in the outer root sheath cells near the upper end of the club.
TS28: hair follicle	Present	Regionally restricted	5C , 5D	Expression was detected in a peg beneath the club in close contact with the follicular papilla.

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present	Regionally restricted	6A , 6B	Nuclear expression was detected in the germ cells growing downwards into the dermis and enveloping the follicular papillae.
TS28: hair outer root sheath	Trace		6A , 6B

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present	Regionally restricted	6D , 6C	As a new hair bulb formed, nuclear staining was detected in basal keratinocytes of the developing outer root sheath and matrix.
TS28: hair root sheath matrix	Present	Regionally restricted	6D , 6C	As a new hair bulb formed, nuclear staining was detected in basal keratinocytes of the developing outer root sheath and matrix.