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Gene Expression Data
Assay Details
Assay
Reference: J:31483 Park CH, et al., Expression of multiple forms of 3 beta-hydroxysteroid dehydrogenase in the mouse liver during fetal and postnatal development. Mol Cell Endocrinol. 1996 Feb 5;116(2):157-64
Assay type: RT-PCR
MGI Accession ID: MGI:3831122
Gene symbol: Hsd3b5
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Probe: primer pair 2
Visualized with: Ethidium bromide
Assay notes: The primers used amplified two family members, Hsd3b4 and Hsd3b5. (This amplicon is present at 649 bp.)
Results Image: 4a
 Sample Information Bands Other Sample Information
Lane AgeStructure 649 bp Amount Genetic BackgroundMutant Allele(s)Sex
40 Female P40 TS28: liver Absent 0.2 µg; total RNA C57BL/6J Female
50 Female P40 TS28: liver Absent 0.2 µg; total RNA C57BL/6J Female
40 Male P50 TS28: liver Present 0.2 µg; total RNA C57BL/6J Male
50 Male P50 TS28: liver Present 0.2 µg; total RNA C57BL/6J Male
-RT Control
S P TS28: spleen Not Specified 0.2 µg; total RNA C57BL/6J Not Specified
H2O Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory