Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18156
Sretavan DW, et al., Embryonic neurons of the developing optic chiasm express L1 and CD44, cell surface molecules with opposing effects on retinal axon growth. Neuron. 1994 May;12(5):957-75
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3830692
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Gene symbol:
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L1cam
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Gene name:
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L1 cell adhesion molecule
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Antibody:
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anti-L1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
3A:
embryonic day 11.5
(more  )
Note: Whole mount of ventral diencephalon.
Specimen
3A:
(close  )
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Genetic Background:
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either: C3H or C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Whole mount of ventral diencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3A
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Immunopositive axons in region of future optic chiasm are arranged in a V shape.
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Specimen
3B:
embryonic day 12.5
(more )
Note: Whole mount of ventral diencephalon.
Specimen
3B:
(close )
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Genetic Background:
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either: C3H or C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Whole mount of ventral diencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3B
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| TS20: optic chiasma |
Present |
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3B
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Immunopositive axons in region of future optic chiasm
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| TS20: optic stalk |
Present |
Regionally restricted |
3B
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Expression in retinal ganglion cell axons which have begun to invade the ventral diencephalon. A few immunopositive axons appear to be leaving the optic nerves to grow toward the future chiasm, but have yet to reach the chiasm neurons.
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Specimen
3C:
embryonic day 13.5
(more )
Note: Whole mount of ventral diencephalon.
Specimen
3C:
(close )
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Genetic Background:
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either: C3H or C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Whole mount of ventral diencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
3C
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| TS21: optic chiasma |
Present |
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3C
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Immunopositive axons in region of future optic chiasm.
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| TS21: optic stalk |
Present |
Regionally restricted |
3C
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A large number of immunopositive retinal ganglion cell axons have grown into the diencephalon and are intermixed with chiasm neurons.
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