Wiz RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:50547 Matsumoto K, et al., Molecular cloning and distinct developmental expression pattern of spliced forms of a novel zinc finger gene wiz in the mouse cerebellum. Brain Res Mol Brain Res. 1998 Oct 30;61(1-2):179-89
Assay type:	RNA in situ
MGI Accession ID:	MGI:3830649
Gene symbol:	Wiz
Gene name:	widely-interspaced zinc finger motifs

Probe:	Wiz original clone
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

Specimen 3B E14: embryonic day 14.0 (more

)

Structure	Level	Image
TS22: brain	Present	3B Probe SL E14
TS22: diencephalon	Present	3B Probe SL E14
TS22: main olfactory bulb	Present	3B Probe SL E14
TS22: cerebral cortex	Strong	3B Probe SL E14
TS22: midbrain	Strong	3B Probe SL E14

Specimen 3B E16: embryonic day 16.0 (more

)

Structure	Level	Image
TS24: brain	Present	3B Probe SL E16
TS24: main olfactory bulb	Present	3B Probe SL E16
TS24: basal ganglia	Strong	3B Probe SL E16
TS24: cerebral cortex	Strong	3B Probe SL E16
TS24: midbrain	Strong	3B Probe SL E16
TS24: cerebellum	Strong	3B Probe SL E16 , 3B Probe SL E16 inset

Specimen 3B P1: postnatal day 1 (more

)

Structure	Level	Image
TS27: brain	Present	3B Probe SL P1
TS27: main olfactory bulb	Present	3B Probe SL P1
TS27: cerebellum	Strong	3B Probe SL P1 , 3B Probe SL P1 inset
TS27: basal ganglia	Strong	3B Probe SL P1 , 3B Probe SL P1 inset
TS27: cerebral cortex	Strong	3B Probe SL P1 , 3B Probe SL P1 inset
TS27: midbrain	Strong	3B Probe SL P1 , 3B Probe SL P1 inset

Specimen 3B P7: postnatal day 7 (more

)

Structure	Level	Image
TS28: brain	Present	3B Probe SL P7
TS28: cerebellum	Strong	3B Probe SL P7 inset , 3B Probe SL P7
TS28: main olfactory bulb	Strong	3B Probe SL P7 inset , 3B Probe SL P7
TS28: cerebral cortex	Weak	3B Probe SL P7 , 3B Probe SL P7 inset
TS28: dentate gyrus	Weak	3B Probe SL P7 , 3B Probe SL P7 inset
TS28: inferior colliculus	Weak	3B Probe SL P7 , 3B Probe SL P7 inset

Specimen 3B Ad: postnatal adult (more

)

Structure	Level	Image	Note
TS28: brain	Present	3B Probe SL Ad
TS28: cerebellum	Present	3B Probe SL Ad
TS28: cerebellum granule cell layer	Present	3B Probe SL Ad
TS28: dentate gyrus	Present	3B Probe SL Ad
TS28: main olfactory bulb	Present	3B Probe SL Ad
TS28: caudate-putamen	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: cerebral cortex	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: hippocampus CA1	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: hippocampus CA2	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: hippocampus CA3	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: hypothalamus	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.
TS28: midbrain	Ambiguous	3B Probe SL Ad	Most of the hybridization signals had disappeared.

Specimen 4A, 4B, 4C, 4D: postnatal day 10 (more

)
Note: Figs. 4B-D are higher magnification images of Fig. 4A.

Structure	Level	Image
TS28: brain subventricular zone	Present	4A
TS28: cerebellum	Present	4A
TS28: hippocampus	Present	4A
TS28: main olfactory bulb	Present	4A
TS28: cerebellum external granule cell layer	Strong	4B , 4A
TS28: cerebellum internal granule cell layer	Strong	4B , 4A
TS28: main olfactory bulb granule cell layer	Weak	4A , 4C
TS28: main olfactory bulb subependymal zone	Present	4A , 4C
TS28: dentate gyrus	Present	4A , 4D

Specimen 5A: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	5A	Expression was detected in the external granule layer.

Specimen 5B: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5B

Specimen 5C: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5C

Specimen 5D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		5D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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