Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Prom1tm1(cre/ERT2)Gilb
Name:	prominin 1; targeted mutation 1, Richard J Gilbertson
MGI ID:	MGI:3830624
Synonyms:	CD133-CreERT2, Prom1C-L
Gene:	Prom1  Location: Chr5:44150962-44259374 bp, - strand  Genetic Position: Chr5, 23.97 cM
Alliance:	Prom1tm1(cre/ERT2)Gilb page

Germline Transmission:	Earliest citation of germline transmission: J:144215
Parent Cell Line:	CMTI-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Prom1tm1(cre/ERT2)Gilb expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Prom1 (MGI:1100886)   Mutation details: The ATG start site of the gene locus was replaced by a cassette containing a creERT2 fusion gene, an IRES-LacZ gene, and a FRT-flanked neomycin selection cassette. Correct targeting was confirmed by PCR. The neomycin selection cassette was later removed by crossing mice with Gt(ROSA)26Sortm1(FLP1)Dym mice. Beta-gal expression was detected in the neural tube, rib, kidney, and gut of E10.5-E16.5 heterozygote embryos. Beta-gal expression was also detected in numerous adult tissues including brain, kidney, retina, pancreas, and intestine. Cre expression was also detected in brain, kidney, lung, pancreas, and intestinal epithelium of heterozygote adult mice after tamoxifen administration. Inactivation of the endogenous gene locus was confirmed by RT-PCR of adult homozygous kidney. (J:144215)

Activity:	Tissue activity of this recombinase allele
Driver:	Prom1 (mouse) Summary of all recombinase alleles driven by Prom1. Alliance mouse Prom1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

List all tumor models in MMHCdb carrying Prom1tm1(cre/ERT2)Gilb

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prom1 Mutation:	75 strains or lines available

Original:	J:144215 Zhu L, et al., Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature. 2009 Jan 29;457(7229):603-7
All:	27 reference(s)