Gene Expression Data
Assay Details
Assay
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Reference:
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J:49822
Bolin LM, et al., HNMP-1: a novel hematopoietic and neural membrane protein differentially regulated in neural development and injury. J Neurosci. 1997 Jul 15;17(14):5493-502
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3830052
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Gene symbol:
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Emp3
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Gene name:
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epithelial membrane protein 3
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Antibody:
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HNMP-1 antibody
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
6A:
embryonic day 12.0
(more )
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
6A
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Expression was detected in a lattice-like pattern of fibers that course from pia mater through differentiating neurons to the central canal.
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TS20: spinal cord floor plate |
Strong |
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6A
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Specimen
not shown:
embryonic day 16.0
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Regionally restricted |
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Expression was detected in the somas of alpha-motoneurons in the ventral spinal cord as well as in the cores of fibers in the developing white matter.
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Specimen
6C:
postnatal adult
(more )
Specimen
6C:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord |
Present |
Regionally restricted |
6C
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Expression was detected in the alpha-motoneurons.
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TS28: spinal cord white matter |
Present |
Regionally restricted |
6C
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Expression was axon-associated in fiber tracts of the white matter.
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Specimen
6D:
embryonic day 12.0
(more )
Specimen
6D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: dorsal root ganglion |
Present |
Regionally restricted |
6D
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Expression was detected in a small subset of cells whose somas and cell processes were positive.
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Specimen
6F:
postnatal adult
(more )
Specimen
6F:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: dorsal root ganglion |
Present |
Regionally restricted |
6F
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Expression was detected in the lumbar 4 dorsal root ganglion in many large sensory neurons and articulating fibers.
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Specimen
8A:
postnatal adult
(more )
Specimen
8A:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: sciatic nerve |
Present |
Regionally restricted |
8A
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Expression was axon-associated with undulating positive fibers that were tangentially sectioned.
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Specimen
8D:
postnatal adult
(more )
Specimen
8D:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: sciatic nerve |
Present |
Regionally restricted |
8D
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In cross section, expression was detected in the axon core, whereas surrounding myelin sheaths were uniformly negative.
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