Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pthlhtm1Broa
Name:	parathyroid hormone-like peptide; targeted mutation 1, Arthur E Broadus
MGI ID:	MGI:3829430
Synonyms:	PTHrP-lacZ
Gene:	Pthlh  Location: Chr6:147153607-147165511 bp, - strand  Genetic Position: Chr6, 78.19 cM, cytoband F-G
Alliance:	Pthlhtm1Broa page

Germline Transmission:	Earliest citation of germline transmission: J:128121
Parent Cell Line:	W9.5/W95 (ES Cell)
Strain of Origin:	129S1/Sv-Oca2+ Tyr+ Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: Exon 3 was replaced with a LacZ reporter cassette. The lacZ cassette encoded the beta-gal coding region preceded by stop codons (TAG) in all three reading frames. The construct also uses a polyA sequence to replace the untranslated region of the gene locus, which contains AU-rich instability sequences. Beta-gal activity was detected in numerous tissues of heterozygous embryos including the mammary bud, inner ear, nasal epithelium, and in the endochondral skeleton. Beta-gal activity was also observed in tendon insertion sites of adult mice and within descrete populations of chondrocytes. (J:128121)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	50 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pthlh Mutation:	19 strains or lines available

Original:	J:128121 Chen X, et al., Initial characterization of PTH-related protein gene-driven lacZ expression in the mouse. J Bone Miner Res. 2006 Jan;21(1):113-23
All:	15 reference(s)