Gene Expression Data
Assay Details
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Assay
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Reference:
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J:143681
Dentice M, et al., Missense mutation in the transcription factor NKX2-5: a novel molecular event in the pathogenesis of thyroid dysgenesis. J Clin Endocrinol Metab. 2006 Apr;91(4):1428-33
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3829275
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Gene symbol:
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Nkx2-1
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Gene name:
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NK2 homeobox 1
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Antibody:
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anti-TTF-1
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Detection system:
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horseradish peroxidase
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Results
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Specimen
WT (bottom):
embryonic day 9.5
(more  )
Specimen
WT (bottom):
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: thyroid primordium |
Present |
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1_WT (bottom) Nkx2-1
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Expression in endodermal cells fated to become thyroid follicular cells.
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Specimen
Nkx2-5 -/- (middle):
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Nkx2-5tm1Siz/Nkx2-5tm1Siz
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: thyroid primordium |
Present |
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1_Nkx2-5-/- (middle) Nkx2-1
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Authors report a reduction in the number of expressing cells compared with wild type.
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Specimen
Nkx2-5 -/- (top):
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Nkx2-5tm1Siz/Nkx2-5tm1Siz
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: thyroid primordium |
Present |
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1_Nkx2-5-/- (top) Nkx2-1
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Authors report a reduction in the number of expressing cells compared with wild type.
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Analysis Tools
