Probe:	shyc EcoRI/Asp718
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Two probes were described, but the probe used in the figures was not detailed (MGI:3822137 and MGI:3822138). However, authors state that both probes yielded the same results.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	2B , 2A	Expression was detected in all germ layers of the embryo. Accumulation of silver grains was observed over the head fold.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	2D , 2C	Expression was strongest as the edges of the neural fold.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted	2F , 2E	Hybridization intensity over the mesoderm was increased.

Structure	Level	Pattern	Image	Note
TS22: peripheral nervous system ganglion	Present		2G , 2H
TS22: central nervous system	Weak		2G , 2H
TS22: head mesenchyme	Strong	Regionally restricted	2G , 2H	Expression was strong in the facial mesoderm.
TS22: gut	Strong		2H , 2G
TS22: lung	Strong		2H , 2G
TS22: skeletal muscle	Strong		2H , 2G
TS22: liver	Absent		2G , 2H

Structure	Level	Pattern	Image	Note
TS24: peripheral nervous system	Present		2I , 2J
TS24: tooth	Present		2I , 2J

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Strong	Regionally restricted	3A , 3B	Expression was strong in the mitral cell layer of the olfactory bulb.
TS24: brain ventricular layer	Strong		3A , 3B
TS24: cortical plate	Strong		3A , 3B
TS24: hippocampus	Strong	Regionally restricted	3A , 3B	Expression was strong in the pyramidal cells of the hippocampal formation.

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus granule cell layer	Present		3C
TS27: hippocampal formation	Present		3C

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus granule cell layer	Present		3D , 3E
TS27: hippocampal formation	Present		3D , 3E

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		4A
TS28: dentate gyrus granule cell layer	Present		4A
TS28: hippocampal formation	Present		4A
TS28: main olfactory bulb granule cell layer	Present		4A
TS28: telencephalon septum	Present	Regionally restricted	4A	Expression was detected in the lateral septum.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4B
TS28: cerebral cortex	Present		4B
TS28: dentate gyrus granule cell layer	Present		4B
TS28: hippocampal formation	Present		4B
TS28: main olfactory bulb granule cell layer	Present		4B
TS28: telencephalon septum	Present	Regionally restricted	4B	Expression was detected in the lateral septum.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		4C , 4D
TS28: hippocampal formation	Present		4C , 4D

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4E , 4F