Probe:	mf1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Sections were fixed in acetone and subsequently in 4% formaldehyde.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present		3a
TS20: ganglionic eminence	Present		3a
TS20: telencephalon ventricular layer	Present		3a
TS20: midbrain ventricular layer	Present	Regionally restricted	3a	Expression is in the posterior mesencephalon.
TS20: retina	Weak	Regionally restricted	3a	Expression is in the outermost layer.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	3b	Expression is at the peripheral region.

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present	Regionally restricted	3c	Expression is at the level of the lateral ventricles.
TS28: choroid plexus	Present	Regionally restricted	3c	Expression is at the level of the lateral ventricles.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		3d

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Strong		4a
TS22: ganglionic eminence	Strong		4a
TS22: intestine	Strong		4a
TS22: liver	Strong		4a
TS22: telencephalon ventricular layer	Strong		4a
TS22: thymus primordium	Strong		4a
TS22: integumental system	Strong	Regionally restricted	4a	Expression is in small islands of brown adipose tissue around the neck and back.
TS22: dorsal root ganglion	Present		4a
TS22: heart	Present		4a
TS22: lung	Present		4a
TS22: midbrain	Present	Regionally restricted	4a	Expression is posterior.
TS22: medulla oblongata	Weak		4a
TS22: spinal cord	Weak		4a

Structure	Level	Pattern	Image	Note
TS24: brown fat	Present		4b

Structure	Level	Pattern	Image	Note
TS28: renal cortex	Present		4c

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present			Throughout development expression persists in the ventricular zone and in the ependymal layer.

Structure	Level	Pattern	Image	Note
TS17: liver	Strong			Expression is in the liver from E10.5 onwards.

Structure	Level	Pattern	Image	Note
TS26: brown fat	Strong

Structure	Level	Pattern	Image	Note
TS28: spleen	Present
TS28: thymus cortex	Present
TS28: brown fat	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent