Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Zbtb7btm2Litt
Name:	zinc finger and BTB domain containing 7B; targeted mutation 2, Dan R Littman
MGI ID:	MGI:3818473
Synonyms:	ThPOK-GFP
Gene:	Zbtb7b  Location: Chr3:89284953-89300976 bp, - strand  Genetic Position: Chr3, 39.09 cM, cytoband A1-A2
Alliance:	Zbtb7btm2Litt page

Germline Transmission:	Earliest citation of germline transmission: J:141519
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: Exons 2 and 3 were replaced with enhanced green fluorescent protein (EGFP) coding sequence followed by the SV40 polyadenlyation signal. During the gene targeting, a loxP-flanked PGK-neomycin cassette was inserted approximately 1.1 kb upstream of the initiation codon in the sense orientation. The PGK-neomycin cassette was deleted in vivo by crossing to EIIa-cre mice and a single loxP site remains. In T lymphocytes of heterozygotes, GFP is specifically expressed in CD4 cells. In homozygotes, B220+ cells also express GFP. (J:141144, J:141519)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Zbtb7b Mutation:	24 strains or lines available

Original:	J:141519 Littman DR, Generation of a Zbtb7b GFP knock-in allele. MGI Direct Data Submission. 2008;
All:	10 reference(s)