Probe:	ish
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were hydrolyzed to approximately 150 bases. It is not clear whether the embryos shown in Figure 5 are d13.5 or d16.5, the text refers to both.

Structure	Level	Pattern	Image	Note
TS14: embryo	Present		3_day 8.5-9.5
TS14: extraembryonic component	Present	Regionally restricted	3_day 8.5-9.5	Expression is in the ectoplacental cone.
TS28: placenta	Present		3_day 8.5-9.5	Expression is in the placenta and the placental membranes.

Structure	Level	Pattern	Image	Note
TS28: uterus	Present	Regionally restricted	3_day 8.5-9.5	Expression is in the mesometrial and antimesometrial tissues and uterine tissues.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present		3_day 13.5
TS21: liver	Strong		3_day 13.5

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		3_day 16.5
TS24: liver	Strong		3_day 16.5

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present		4A
TS14: somite	Present		4A

Structure	Level	Pattern	Image	Note
TS14: cardiac muscle tissue	Present		4B
TS14: heart	Present	Regionally restricted	4B	Expression is in the endocardium.

Structure	Level	Pattern	Image	Note
TS21: tibia-fibula pre-cartilage condensation	Present	Regionally restricted	5A	Expression is in chondrocytes and perichondrial cells.

Structure	Level	Pattern	Image	Note
TS21: chondrocranium	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS21: mandible	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS21: maxilla	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS21: cartilage	Present	Regionally restricted		Expression is in perichondrial cells and chondrocytes of articular cartilage.
TS21: limb	Present	Regionally restricted		Expression is in the presumptive growth plate and the cartilage model within the central canal.
TS21: blood vessel	Present	Regionally restricted		Expression is in blood vessel endothelial cells withing the mid-diaphysis.
TS21: skeletal muscle	Present	Regionally restricted		Expression is in the skeletal muscle cells surrounding the diaphysis.
TS21: arterial system	Present	Regionally restricted		Expression is in the walls of the large arteries.
TS21: liver parenchyma	Present
TS21: brainstem	Strong
TS21: peripheral nervous system	Strong
TS21: spinal cord	Strong
TS21: telencephalon	Strong

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		5B upper panel
TS21: lung mesenchyme	Present		5B upper panel

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		5B lower panel
TS21: endocardial cushion	Present		5B lower panel

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Regionally restricted	5C upper panel	Expression is in acinar epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: intestine epithelium	Present	Regionally restricted	5C lower panel	Expression is in the epithelial and subepithelial cells.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present	Regionally restricted	5D upper panel	Expression is in the developing nephrons, kidney blastema, and kidney interstitium.
TS21: ureter	Present	Regionally restricted	5D upper panel	Expression is in the ureteric bud.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted	5D lower panel	Expression is in epidermal and adnexal keratinocytes, dermal connective tissue, and brown fat.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	5E	Expression is in ganglion cells.

Structure	Level	Pattern	Image	Note
TS24: chondrocranium	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS24: mandible	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS24: maxilla	Present	Regionally restricted		Expression is in osteoblasts and periosteal cells.
TS24: cartilage	Present	Regionally restricted		Expression is in perichondrial cells and chondrocytes of articular cartilage.
TS24: limb	Present	Regionally restricted		Expression is in the presumptive growth plate and the cartilage model within the central canal.
TS24: blood vessel	Present	Regionally restricted		Expression is in blood vessel endothelial cells withing the mid-diaphysis.
TS24: skeletal muscle	Present	Regionally restricted		Expression is in the skeletal muscle cells surrounding the diaphysis.
TS24: cardiac muscle tissue	Present
TS24: endocardial cushion	Present
TS24: liver parenchyma	Present
TS24: lung epithelium	Present
TS24: lung mesenchyme	Present
TS24: maturing nephron	Present
TS24: pancreas	Present	Regionally restricted		Expression is in acinar epithelial cells.
TS24: intestine epithelium	Present	Regionally restricted		Expression is in the epithelial and subepithelial cells.
TS24: lower leg	Present	Regionally restricted		Expression is in cartilage and bone.
TS24: arterial system	Present	Regionally restricted		Expression is in the walls of the large arteries.
TS24: metanephros	Present	Regionally restricted		Expression is in the kidney blastema and kidney interstitium.
TS24: ureter	Present	Regionally restricted		Expression is in the ureteric bud.
TS24: skin	Present	Regionally restricted		Expression is in epidermal and adnexal keratinocytes, dermal connective tissue, and brown fat.
TS24: retina	Present	Regionally restricted		Expression is in ganglion cells.
TS24: brainstem	Strong
TS24: peripheral nervous system	Strong
TS24: spinal cord	Strong
TS24: telencephalon	Strong