Antibody:	clone RC2
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: hindbrain floor plate	Present	Non-Uniform	2C , 2D	Expression is particularly strong in the cell-body-free zone.
TS20: hindbrain	Weak		2C , 2D	Weak expression is detected in the radial glia fibers throughout the hindbrain region.
TS20: hindbrain marginal layer	Present		2C , 2D	Expression is enriched in the marginal zone where the radial glia end feet are located.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	2L , 2K , 2J	Expression is present in midline glial raphe.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2T , 2S , 2R	Expression is present throughout the developing cortex, where it is localized in radial glia.