Antibody:	anti-ABBA
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Structure	Level	Pattern	Image	Note
TS20: hindbrain floor plate	Present	Non-Uniform	2B , 2D	Expression is particularly strong in the cell-body-free zone.
TS20: hindbrain	Weak		2B , 2D	Weak expression is detected in the radial glia fibers throughout the hindbrain region
TS20: hindbrain marginal layer	Present		2D , 2B	Expression is enriched in the marginal zone where the radial glia end feet are located.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		2E , 2G , 2H	Expression is present in glia.
TS20: spinal cord	Present	Regionally restricted	2E , 2G , 2H	Although expression is found elsewhere in the spinal cord, it is not found in neurons.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	2I , 2L , 2K	Expression is present in midline glial raphe.

Structure	Level	Pattern	Image	Note
TS22: brain	Present	Regionally restricted	2O , 2M , 2P	Expression is present in midline glial raphe. It is not present in the surrounding neuronal tissue.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	2S , 2Q , 2T	Expression is present throughout the developing cortex, where it is localized in radial glia. It is particularly strong in the radial glial end feet area, next to the pial surface.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present			The highest expression levels in the brain are seen here, in both the Bergmann glia and in the tightly associated Purkinje cell extensions.