Probe:	pBSIIKS-ABBA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS16: floor plate	Present			Expression is detected only in the floorplate at the ventral midline of the neural tube from the midbrain until the end of the spinal cord.
TS16: future hindbrain floor plate	Present			Expression is detected only in the floorplate at the ventral midline of the neural tube from the midbrain until the end of the spinal cord.
TS16: future midbrain floor plate	Present			Expression is detected only in the floorplate at the ventral midline of the neural tube from the midbrain until the end of the spinal cord.
TS16: neural tube floor plate	Present			Expression is detected only in the floorplate at the ventral midline of the neural tube from the midbrain until the end of the spinal cord.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Strong		1C
TS20: spinal cord	Present	Regionally restricted	1C	Expression is detected in parenchyma.
TS20: spinal cord marginal layer	Present	Regionally restricted	1C	Expression is detected in the outer border of the marginal zone where radial glia endplates locate.

Structure	Level	Pattern	Image	Note
TS20: hindbrain floor plate	Strong		1F
TS20: hindbrain	Present		1F	Expression is in the parenchyma.
TS20: hindbrain marginal layer	Present	Regionally restricted	1F	Expression is present in the outer border of the marginal zone where radial glia endplates locate.

Structure	Level	Pattern	Image	Note
TS20: skeleton	Present			Expression is present in the developing bone.
TS20: skeletal muscle	Present

Structure	Level	Pattern	Image	Note
TS22: brain pia mater	Strong	Regionally restricted	1G	Expression is detected in the pial surface surrounding the brain.
TS22: brain	Strong	Regionally restricted	1G	Expression is detected in the midline glial raphe.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		1H
TS22: cerebral cortex marginal layer	Present	Non-Uniform	1H	There is especially strong expression in the outer border of the marginal zone.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	1I	Expression is present in the brainstem raphe.

Structure	Level	Pattern	Image	Note
TS26: brainstem	Present	Regionally restricted	1J	Expression is present in the brainstem raphe.
TS26: brain pia mater	Present	Regionally restricted	1J	Expression is present in the pial surface.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Present		1K	This is the strongest expression seen in the adult brain.

Structure	Level	Pattern	Image	Note
TS28: brain	Moderate	Non-Uniform		Moderate expression is present throughout the brain (except in the cerebellar molecular layer, where it is stronger).
TS28: lung	Moderate
TS28: skeletal muscle	Moderate
TS28: testis	Moderate
TS28: heart	Weak
TS28: liver	Weak
TS28: metanephros	Weak