Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:108509 Blaess S, et al., Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region. Development. 2006 May;133(9):1799-809
Assay type:	Western blot
MGI Accession ID:	MGI:3810924
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:

anti-Gli3

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex	Note
Shh +/+	E12.5	TS20: cerebellum primordium TS20: midbrain	Present	Present	Not Specified		Not Specified	mesencephalon and rhombomere 1
Shh +/-	E12.5	TS20: cerebellum primordium TS20: midbrain	Present (c)	Present	involves: 129S1/Sv * 129X1/SvJ * Swiss Webster	Shh^tm1Chg/Shh⁺	Not Specified	mesencephalon and rhombomere 1
Shh -/-	E12.5	TS20: brain	Present (d)	Present (e)	involves: 129S1/Sv * 129X1/SvJ * Swiss Webster	Shh^tm1Chg/Shh^tm1Chg	Not Specified

Notes:
(a) This band represents the full length form.
(b) This band represents the N-terminal repressor form.
(c) Expression levels are slightly reduced compared to what is seen in wild type.
(d) The expression level of this form is reduced relative to what was seen in wild type.
(e) The expression level of this form is elevated relative to what was seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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