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Gene Expression Data
Assay Details
Assay
Reference: J:29276 Acampora D, et al., Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-90
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3810315
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Reporter: lacZ
Direct Detection
Results
Specimen 2a: embryonic day 5.75; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 2a
TS8: epiblast Weak 2a
Specimen 2b: embryonic day 5.75; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 2b
TS8: epiblast Weak Spotted 2b
Specimen 2e: embryonic day 7.0; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS10: embryo endoderm Present Regionally restricted 2e Expression is in the external layer of the endomesoderm in the anterior part.
TS10: embryo mesoderm Present Regionally restricted 2e Expression is in the external layer of the endomesoderm in the anterior part.
TS10: non-neural ectoderm Absent 2e
Specimen 2f: embryonic day 6.5; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS9: embryo endoderm Present Regionally restricted 2f Expression is in the external layer of the endomesoderm in the anterior part.
TS9: embryo mesoderm Present Regionally restricted 2f Expression is in the external layer of the endomesoderm in the anterior part.
TS9: embryo ectoderm Absent 2f
Specimen 2g: embryonic day 6.5; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS9: embryo endoderm Present 2g Expression is in the external layer of the endomesoderm in the anterior part.
TS9: embryo mesoderm Present 2g Expression is in the external layer of the endomesoderm in the anterior part.
TS9: embryo ectoderm Absent 2g
Specimen 2j: embryonic day 7.0; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS10: embryo endoderm Present Regionally restricted 2j Expression is in the external layer of the endomesoderm in the anterior part.
TS10: embryo mesoderm Present Regionally restricted 2j Expression is in the external layer of the endomesoderm in the anterior part.
TS10: non-neural ectoderm Absent 2j
Specimen 2k: embryonic day 7.75; Otx2tm1Pas/Otx2+ (more )
Note: Head-fold stage.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 2k Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
TS11: embryo endoderm Present Regionally restricted 2k Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
TS11: embryo mesoderm Present Regionally restricted 2k Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
Specimen 2l: embryonic day 7.75; Otx2tm1Pas/Otx2+ (more )
Note: Head-fold stage.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 2l Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
TS11: embryo endoderm Present Regionally restricted 2l Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
TS11: embryo mesoderm Present Regionally restricted 2l Expression is in the anterior part of all three germ layers in the most anterior one-third of the embryos.
TS11: embryo Present Regionally restricted 2l In the midline, a strong staining was also present at the oral plate level, immediately adjacent to the embryonic-extraembryonic junction.
Specimen 2o: embryonic day 9.25; Otx2tm1Pas/Otx2+ (more )
Note: 16-20 somites stage.
Structure Level Pattern Image Note
TS14: foregut Present 2o
TS14: future midbrain Present 2o
TS14: prosencephalon Present 2o
Specimen 2p: embryonic day 9.25; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS14: thyroid primordium Strong 2p
TS14: 1st branchial arch Present 2p
TS14: olfactory placode Present 2p
TS14: head Present Regionally restricted 2p Expression is in the rostral-most head ectoderm.
Specimen 2q: embryonic day 9.25; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS14: notochord Strong Regionally restricted 2q Expression is in the anterior half of the notochord.
TS14: head Present Regionally restricted 2q Expression is in the rostral-most head ectoderm.
TS14: 1st branchial arch Present 2q
TS14: olfactory placode Present 2q
Specimen 2r: embryonic day 10.5; Otx2tm1Pas/Otx2+ (more )
Structure Level Pattern Image Note
TS17: eye Present 2r
TS17: midbrain Present 2r
TS17: oral epithelium Present 2r
TS17: forebrain Present Regionally restricted 2r Expression is in the forebrain and is absent in the optic recess and optic chiasma.
TS17: hindbrain Absent 2r
Specimen 5a: embryonic day 6.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 5a Expression is in the distal half of the embryonic part.
Specimen 5b: embryonic day 6.5; Otx2tm1Pas/Otx2tm1Pas (more )
Note: (b) Sagittal section of the embryo shown in a.
Structure Level Pattern Image Note
TS9: embryo endoderm Present Regionally restricted 5b Expression is in the external layer containing endomesoderm cells.
TS9: embryo mesoderm Present Regionally restricted 5b Expression is in the external layer containing endomesoderm cells.
Specimen 6a: embryonic day 7.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS11: surface ectoderm Weak 6a
Specimen 6b: embryonic day 7.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS11: surface ectoderm Weak 6b
Specimen 6c: embryonic day 7.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS11: surface ectoderm Strong Regionally restricted 6c Expression is in a limited domain in the anterior part.
Specimen Not shown: embryonic day 8.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS13: foregut diverticulum Absent
Specimen Not shown: embryonic day 9.5; Otx2tm1Pas/Otx2tm1Pas (more )
Structure Level Pattern Image Note
TS15: foregut Absent
TS15: head Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/20/2026
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