Gt(ROSA)26Sortm14(CAG-tdTomato)Hze
Targeted Allele Detail
|
Symbol: |
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Hongkui Zeng |
MGI ID: |
MGI:3809524 |
Synonyms: |
Ai14, Ai4, Gt(ROSA)26Sor_tm14(CAG/LSL_tdTomato)Hze, Gt(ROSA)26Sortm14(CAT-tdTomato)Hze, Gt(ROSA)26Sortm9.1(CAG-tdTomato)Hze, R26-RFP, R26RT, R26Tom, RCL-tdT, Rosa26Tdt, Rosa26-tdTomato, Rosa-CAG-LSL-tdTomato-WPRE::deltaNeo, Rosa-LSL.dTm, Rosa-lsl-tdTomato, STOP-RFP, tdTomato, Tomatoflox |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
|
Alliance: |
Gt(ROSA)26Sortm14(CAG-tdTomato)Hze page
|
|
|
Allele Type: |
|
Targeted (Conditional ready, Reporter) |
Mutation: |
|
Insertion
|
|
|
Mutation details: The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. phiC31-recombination of Gt(ROSA)26Sortm9(CAG-tdTomato)Hze removed the neomycin resistance cassette.
(J:155793)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
|
|
Original: |
J:155793 Madisen L, et al., A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010 Jan;13(1):133-40 |
All: |
2423 reference(s) |
|