Gene Expression Data
Assay Details
Assay
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Reference:
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J:79874
Nguyen TB, et al., Characterization and expression of Mammalian cyclin b3, a prepachytene meiotic cyclin. J Biol Chem. 2002 Nov 1;277(44):41960-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3806863
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Gene symbol:
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Ccnb3
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Gene name:
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cyclin B3
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Probe:
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cyclin B3 cDNA
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
Not shown:
postnatal day 7.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 7.5
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Sex:
|
Male
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Type:
|
section
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Embedding:
|
Paraffin
|
Specimen
9 PND8.5 (dark field):
postnatal day 8.5
(more )
Specimen
9 PND8.5 (dark field):
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 8.5
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Sex:
|
Male
|
Type:
|
section
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
9_PND8.5 as dark field
|
Rare tubules show expression. This expression is in the center of the tubule. No expression is seen in undeveloped tubules that contain only spermatogonia and Steroli cells.
|
Specimen
9 PND9.5 (dark field):
postnatal day 9.5
(more )
Specimen
9 PND9.5 (dark field):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 9.5
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
9_PND9.5 as dark field
|
Some tubules show expression. This expression is in the center of the tubule. No expression is seen in undeveloped tubules that contain only spermatogonia and Steroli cells.
|
Specimen
Not shown:
postnatal day 14.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
|
Some tubules shown expression. This expression is in the center of the tubule. No expression is seen in undeveloped tubules that contain only spermatogonia and Steroli cells.
|
Specimen
9 PND14.5 (dark field):
postnatal day 14.5
(more )
Specimen
9 PND14.5 (dark field):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
9_PND14.5 as dark field
|
Tubules containing leptotene or zygotene spermatocytes showed expression but precocious tubules that had progressed to the pachytene stage did not.
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Specimen
9 PND8.5 (bright field):
postnatal day 8.5
(more )
Specimen
9 PND8.5 (bright field):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 8.5
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
9_PND8.5 as bright field
|
Rare tubules show expression. This expression is in the center of the tubule. No expression is seen in undeveloped tubules that contain only spermatogonia and Steroli cells.
|
Specimen
9 PND14.5 (left):
postnatal day 14.5
(more )
Specimen
9 PND14.5 (left):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14.5
|
Sex:
|
Male
|
Type:
|
section
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Embedding:
|
Paraffin
|
Specimen
9 PND14.5 (center):
postnatal day 14.5
(more )
Specimen
9 PND14.5 (center):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Specimen
9 PND14.5 (right):
postnatal day 14.5
(more )
Specimen
9 PND14.5 (right):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 14.5
|
Sex:
|
Male
|
Type:
|
section
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Embedding:
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Paraffin
|
Specimen
10 adult:
postnatal month 8
(more )
Specimen
10 adult:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 8
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Sex:
|
Male
|
Type:
|
section
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
10_dark field
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Expression is present in tubules between stages VIII and XII. There is no expression in tubles between stages I and VII.
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Specimen
10 st. II:
postnatal month 8
(more )
Specimen
10 st. II:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal month 8
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Sex:
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Male
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Type:
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section
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Embedding:
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Paraffin
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Specimen
10 st. IV:
postnatal month 8
(more )
Specimen
10 st. IV:
(close )
Genetic Background:
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Not Specified
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Age:
|
postnatal month 8
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Sex:
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Male
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Type:
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section
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Embedding:
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Paraffin
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Specimen
10 st. VI-VII:
postnatal month 8
(more )
Specimen
10 st. VI-VII:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal month 8
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Sex:
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Male
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Type:
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section
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Embedding:
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Paraffin
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Specimen
10 st. VIII-IX:
postnatal month 8
(more )
Specimen
10 st. VIII-IX:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal month 8
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Sex:
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Male
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Type:
|
section
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Embedding:
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Paraffin
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Specimen
10 st. X:
postnatal month 8
(more )
Specimen
10 st. X:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal month 8
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Sex:
|
Male
|
Type:
|
section
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
10_st. X
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Expression is present in leptotene and zygotene cells. It is not detected in the pachytene, diplotene, or secondary spermatocytes.
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Specimen
10 st. XI:
postnatal month 8
(more )
Specimen
10 st. XI:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal month 8
|
Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
10_st. XI
|
Expression is present in leptotene and zygotene cells. It is not detected in the pachytene, diplotene, or secondary spermatocytes.
|
Specimen
10 st. XII:
postnatal month 8
(more )
Specimen
10 st. XII:
(close )
Genetic Background:
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Not Specified
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Age:
|
postnatal month 8
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Sex:
|
Male
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
10_st. XII
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Expression is present in leptotene and zygotene cells. It is not detected in the pachytene, diplotene, or secondary spermatocytes.
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Specimen
11A:
embryonic day 15.5
(more )
Specimen
11A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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section
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: ovary |
Present |
Regionally restricted |
11A
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Expression is believed to be in presumptive oocytes.
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Specimen
11C:
embryonic day 15.5
(more )
Specimen
11C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Female
|
Type:
|
section
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: ovary |
Present |
Regionally restricted |
11C
|
Expression is believed to be in presumptive oocytes.
|
Specimen
11B:
postnatal week 3
(more )
Specimen
11B:
(close )
Genetic Background:
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Not Specified
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Age:
|
postnatal week 3
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Sex:
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Female
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Type:
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section
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Embedding:
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Paraffin
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Specimen
11D:
postnatal week 3
(more )
Specimen
11D:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 3
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Sex:
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Female
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Type:
|
section
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Embedding:
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Paraffin
|
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