Pomt2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:137930 Lommel M, et al., POMT2, a key enzyme in Walker-Warburg syndrome: somatic sPOMT2, but not testis-specific tPOMT2, is crucial for mannosyltransferase activity in vivo. Glycobiology. 2008 Aug;18(8):615-25
Assay type:	RT-PCR
MGI Accession ID:	MGI:3806528
Gene symbol:	Pomt2
Gene name:	protein-O-mannosyltransferase 2

Probe:	Pomt2-pA, Pomt2-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR.

Results

Image: 2A Pomt2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
testis	P adult	TS28: testis	Strong (a)	2 µg; total RNA	Not Specified		Male
brain	P adult	TS28: brain	Present (b)	2 µg; total RNA	Not Specified		Not Specified
muscle	P adult	TS28: skeletal muscle	Weak	2 µg; total RNA	Not Specified		Not Specified
kidney	P adult	TS28: metanephros	Present (c)	2 µg; total RNA	Not Specified		Not Specified
liver	P adult	TS28: liver	Present (c)	2 µg; total RNA	Not Specified		Not Specified

Notes:
(a) There was a 50-fold increase in this tissue compared to skeletal muscle.
(b) There was a 2-fold increase in this tissue compared to skeletal muscle.
(c) There was a 5-fold increase in this tissue compared to skeletal muscle.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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