Atp2a1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:98210 Radzyukevich TL, et al., The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10
Assay type:	Western blot
MGI Accession ID:	MGI:3806073
Gene symbol:	Atp2a1
Gene name:	ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

Antibody:	anti-SERCA1
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The antibody used in this assay recognizes both the a and b splice variants.

Results

Image: 7_SERCA1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
WT	E18.5 (a)	TS26: diaphragm	Present (b)	Not Specified		Not Specified
WT	E18.5 (a)	TS26: diaphragm	Present (b)	Not Specified		Not Specified
a2 HET	E18.5 (a)	TS26: diaphragm	Present (c)	involves: 129P2/OlaHsd	Atp1a2^tm2Ling/Atp1a2⁺	Not Specified
a2 HET	E18.5 (a)	TS26: diaphragm	Present (c)	involves: 129P2/OlaHsd	Atp1a2^tm2Ling/Atp1a2⁺	Not Specified
a2 KO	E18.5 (a)	TS26: diaphragm	Present (d)	involves: 129P2/OlaHsd	Atp1a2^tm2Ling/Atp1a2^tm2Ling	Not Specified
a2 KO	E18.5 (a)	TS26: diaphragm	Present (d)	involves: 129P2/OlaHsd	Atp1a2^tm2Ling/Atp1a2^tm2Ling	Not Specified
Lane 7	Lane not used in this assay
WT	P adult	TS28: diaphragm	Present	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels in the embryonic diaphragm are comparable to those seen in the adult.
(c) Expression levels in the heterozygotes are 125% of those seen in wild type.
(d) Expression levels in the homozygotes are 76% of those seen in wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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