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Gene Expression Data
Assay Details
Assay
Reference: J:98210 Radzyukevich TL, et al., The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10
Assay type: Western blot
MGI Accession ID: MGI:3806073
Gene symbol: Atp2a1
Gene name: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Antibody: anti-SERCA1
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used in this assay recognizes both the a and b splice variants.
Results Image: 7_SERCA1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
WT E18.5 (a) TS26: diaphragm Present (b) Not Specified Not Specified
WT E18.5 (a) TS26: diaphragm Present (b) Not Specified Not Specified
a2 HET E18.5 (a) TS26: diaphragm Present (c) involves: 129P2/OlaHsd Atp1a2tm2Ling/Atp1a2+Not Specified
a2 HET E18.5 (a) TS26: diaphragm Present (c) involves: 129P2/OlaHsd Atp1a2tm2Ling/Atp1a2+Not Specified
a2 KO E18.5 (a) TS26: diaphragm Present (d) involves: 129P2/OlaHsd Atp1a2tm2Ling/Atp1a2tm2LingNot Specified
a2 KO E18.5 (a) TS26: diaphragm Present (d) involves: 129P2/OlaHsd Atp1a2tm2Ling/Atp1a2tm2LingNot Specified
Lane 7 Lane not used in this assay
WT P adult TS28: diaphragm Present Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels in the embryonic diaphragm are comparable to those seen in the adult.
(c) Expression levels in the heterozygotes are 125% of those seen in wild type.
(d) Expression levels in the homozygotes are 76% of those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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