Antibody:	SCA7 (335-578)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The sections were fixed in formalin. An identical staining pattern but with more distinct staining was seen with the antibody, SCA7 (1-135) MGI:3805741, as is shown in MGI:3805757.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Strong		4B	Strong nuclear staining.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer II	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer III	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer IV	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer V	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer VI	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cranial nerve	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: dentate gyrus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: entorhinal cortex	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA1	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA2	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA3	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA4	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hypothalamus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: inferior colliculus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: retina ganglion cell layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: retina inner nuclear layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: superior colliculus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: deep cerebellar nucleus	Strong	Regionally restricted		Strong nuclear and moderate cytoplasmic labeling in the dentate nucleus.
TS28: testis	Strong			Strong cytoplasmic and moderate nuclear staining.
TS28: spermatogonium	Moderate
TS28: substantia nigra	Moderate
TS28: bronchiole epithelium	Weak
TS28: cortical layer I	Weak
TS28: liver	Weak
TS28: metanephros	Weak
TS28: photoreceptor layer	Weak
TS28: retina inner plexiform layer	Weak
TS28: retina outer nuclear layer	Weak
TS28: retina outer plexiform layer	Weak
TS28: basal ganglia	Absent
TS28: cerebellum granule cell layer	Absent
TS28: heart	Absent
TS28: spleen	Absent
TS28: thalamus	Absent

Structure	Level	Pattern	Image	Note
TS19: embryo	Weak			There is weak expression in most embryonic tyissues.
TS19: gut	Weak
TS19: heart	Weak
TS19: limb	Weak
TS19: liver	Weak
TS19: lung	Weak
TS19: metanephros	Weak
TS19: cranial nerve	Present
TS19: dorsal root ganglion	Present
TS19: future spinal cord	Present
TS19: retina	Present
TS19: spinal nerve	Present
TS19: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.

Structure	Level	Pattern	Image	Note
TS21: embryo	Weak			There is weak expression in most embryonic tyissues.
TS21: gut	Weak
TS21: heart	Weak
TS21: limb	Weak
TS21: liver	Weak
TS21: lung	Weak
TS21: metanephros	Weak
TS21: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS21: cranial nerve	Present
TS21: dorsal root ganglion	Present
TS21: retina	Present
TS21: spinal cord	Present
TS21: spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS23: embryo	Weak			There is weak expression in most embryonic tyissues.
TS23: gut	Weak
TS23: heart	Weak
TS23: limb	Weak
TS23: liver	Weak
TS23: lung	Weak
TS23: metanephros	Weak
TS23: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS23: cranial nerve	Present
TS23: dorsal root ganglion	Present
TS23: retina	Present
TS23: spinal cord	Present
TS23: spinal nerve plexus	Present

Structure	Level	Pattern	Image	Note
TS25: embryo	Weak			There is weak expression in most embryonic tyissues.
TS25: gut	Weak
TS25: heart	Weak
TS25: limb	Weak
TS25: liver	Weak
TS25: lung	Weak
TS25: metanephros	Weak
TS25: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS25: cranial nerve	Present
TS25: dorsal root ganglion	Present
TS25: retina	Present
TS25: spinal cord	Present
TS25: spinal nerve plexus	Present