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Gene Expression Data
Assay Details
Assay
Reference: J:98851 Porcu S, et al., beta-Minor globin gene expression is preferentially reduced in EKLF Knock-Out mice. Gene. 2005 May 23;351:11-7
Assay type: RT-PCR
MGI Accession ID: MGI:3805812
Gene symbol: Hbb-b1
Gene name: hemoglobin, beta adult major chain
Probe: B exon primer set
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: The primer set used for this assay amplifies two genes. Endonuclease digestion was then used to determine which gene products had been amplified.
Results Image: 2B
 Sample Information Bands Other Sample Information
Lane AgeStructure 351 bp Amount Genetic BackgroundMutant Allele(s)Sex
ND Control
M Mol. Wt. Marker Lane
-/+ E14.5 (a) TS22: liver Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/10 * CBA Klf1tm1Nimr/Klf1+Not Specified
+/+ E14.5 (a) TS22: liver Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/10 * CBA Not Specified
-/- E14.5 (a) TS22: liver Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/10 * CBA Klf1tm1Nimr/Klf1tm1NimrNot Specified
-/- E14.5 (a) TS22: liver Present Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/10 * CBA Klf1tm1Nimr/Klf1tm1NimrNot Specified
ND Control
M Mol. Wt. Marker Lane
Notes:
(a) Age of embryo at noon of plug day not specified in reference.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory