Antibody:	SCA7 (1-135)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The sections were fixed in formalin. This antibody gave the most distinct staining but 2 other antibodies (SCA7 (1-15) MGI:3805739 and SCA7 (335-578) MGI:3805737) gave identical staining patterns (shown in and respectively).

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		3B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3C

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3D

Structure	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	Strong	Regionally restricted	3E	Strong nuclear and moderate cytoplasmic labeling in the dentate nucleus.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Strong	Regionally restricted	3F	Strong nuclear and moderate cytoplasmic labeling.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		3G
TS28: cortical layer II	Present		3G
TS28: cortical layer III	Present		3G
TS28: cortical layer IV	Present		3G
TS28: cortical layer V	Present		3G
TS28: cortical layer VI	Present		3G

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3H

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong	Regionally restricted	3I	Expression is in ganglion cells and in cell bodies of the inner nuclear layer.
TS28: photoreceptor layer outer segment	Weak		3I

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer II	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer III	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer IV	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer V	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cortical layer VI	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: cranial nerve	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: dentate gyrus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: entorhinal cortex	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA1	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA2	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA3	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hippocampus CA4	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: hypothalamus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: inferior colliculus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: islets of Langerhans	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: retina ganglion cell layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: retina inner nuclear layer	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: superior colliculus	Strong			Strong nuclear and moderate cytoplasmic labeling.
TS28: deep cerebellar nucleus	Strong	Regionally restricted		Strong nuclear and moderate cytoplasmic labeling in the dentate nucleus.
TS28: testis	Strong			Strong cytoplasmic and moderate nuclear staining.
TS28: substantia nigra	Moderate
TS28: bronchiole epithelium	Weak
TS28: cortical layer I	Weak
TS28: liver	Weak
TS28: metanephros	Weak
TS28: photoreceptor layer	Weak
TS28: retina inner plexiform layer	Weak
TS28: retina outer nuclear layer	Weak
TS28: retina outer plexiform layer	Weak
TS28: basal ganglia	Absent
TS28: cerebellum granule cell layer	Absent
TS28: thalamus	Absent

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		4A

Structure	Level	Pattern	Image	Note
TS28: heart	Absent
TS28: spleen	Absent

Structure	Level	Pattern	Image	Note
TS19: retina	Present		5B

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		5C
TS19: future spinal cord	Present		5C

Structure	Level	Pattern	Image	Note
TS19: lung	Present		5D

Structure	Level	Pattern	Image	Note
TS19: embryo	Weak			There is weak expression in most embryonic tyissues.
TS19: gut	Weak
TS19: heart	Weak
TS19: limb	Weak
TS19: liver	Weak
TS19: metanephros	Weak
TS19: cranial nerve	Present
TS19: spinal nerve	Present
TS19: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.

Structure	Level	Pattern	Image	Note
TS21: embryo	Weak			There is weak expression in most embryonic tyissues.
TS21: gut	Weak
TS21: heart	Weak
TS21: limb	Weak
TS21: liver	Weak
TS21: lung	Weak
TS21: metanephros	Weak
TS21: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS21: cranial nerve	Present
TS21: dorsal root ganglion	Present
TS21: retina	Present
TS21: spinal cord	Present
TS21: spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS23: embryo	Weak			There is weak expression in most embryonic tyissues.
TS23: gut	Weak
TS23: heart	Weak
TS23: limb	Weak
TS23: liver	Weak
TS23: lung	Weak
TS23: metanephros	Weak
TS23: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS23: cranial nerve	Present
TS23: dorsal root ganglion	Present
TS23: retina	Present
TS23: spinal cord	Present
TS23: spinal nerve plexus	Present

Structure	Level	Pattern	Image	Note
TS25: embryo	Weak			There is weak expression in most embryonic tyissues.
TS25: gut	Weak
TS25: heart	Weak
TS25: limb	Weak
TS25: liver	Weak
TS25: lung	Weak
TS25: metanephros	Weak
TS25: central nervous system	Present	Regionally restricted		Expression is in most parts of the central nervous system.
TS25: cranial nerve	Present
TS25: dorsal root ganglion	Present
TS25: retina	Present
TS25: spinal cord	Present
TS25: spinal nerve plexus	Present