Ccna2 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:108720 Berthet C, et al., Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation. Dev Cell. 2006 May;10(5):563-73
Assay type:	Western blot
MGI Accession ID:	MGI:3803922
Gene symbol:	Ccna2
Gene name:	cyclin A2

Antibody:

Anti-Cyclin A (H-432) sc-751

Results

Image: 4_CycA2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
WT	E13.5 (a)	TS21: embryo	Present	Not Specified		Not Specified
DKO	E13.5 (a)	TS21: embryo	Present (b)	involves: 129S1/Sv * C57BL/6	Cdk2^tm1Kald/Cdk2^tm1Kald, Cdk4^tm1Kiyo/Cdk4^tm1Kiyo	Not Specified
WT	E14.5 (a)	TS22: embryo	Present	Not Specified		Not Specified
DKO	E14.5 (a)	TS22: embryo	Weak (b)	involves: 129S1/Sv * C57BL/6	Cdk2^tm1Kald/Cdk2^tm1Kald, Cdk4^tm1Kiyo/Cdk4^tm1Kiyo	Not Specified
WT	E16.5 (a)	TS24: embryo	Present	Not Specified		Not Specified
DKO	E16.5 (a)	TS24: embryo	Weak (b)	involves: 129S1/Sv * C57BL/6	Cdk2^tm1Kald/Cdk2^tm1Kald, Cdk4^tm1Kiyo/Cdk4^tm1Kiyo	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression level was similar in wild type and double knockout embryos at E13.5, but decreased progressively in double knockout embryos at E14.5 and E16.5 compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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