Hnf1b In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:58079 Barbacci E, et al., Variant hepatocyte nuclear factor 1 is required for visceral endoderm specification. Development. 1999 Nov;126(21):4795-805
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:3798195
Gene symbol:	Hnf1b
Gene name:	HNF1 homeobox B

Reporter:	lacZ
Antibody:	anti-B-galactosidase (Sigma)
Detection system:	Secondary antibody coupled to Texas Red

Results

4E ©

4I ©

Specimen 4E/4I: embryonic day 5.5; Hnf1b^tm1Sce/Hnf1b⁺ (more

)
Note: Double labeled: red - Hnf1b; green - Krt19 (assay MGI:1346730).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present		4I , 4E

4F ©

4J ©

Specimen 4F/4J: embryonic day 5.5; Hnf1b^tm1Sce/Hnf1b^tm1Sce (more

)
Note: Double labeled: red - Hnf1b; green - Krt19 (assay MGI:1346730).

Structure	Level	Pattern	Image	Note
TS7: parietal endoderm	Present	Regionally restricted	4F , 4J	Only a fraction of beta-galactosidase positived cells were parietal endoderm-like cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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