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Gene Expression Data
Assay Details
Assay
Reference: J:74909 Gerety SS, et al., Cardiovascular ephrinB2 function is essential for embryonic angiogenesis. Development. 2002 Mar;129(6):1397-410
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3798171
Gene symbol: Efnb2
Gene name: ephrin B2
Reporter: lacZ
Antibody: anti-B-galactosidase
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 5A, 5B: embryonic day 9.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: red - Efnb2 reporter; green - Pecam1 (assay MGI:2176794).
Structure Level Pattern Image Note
TS15: future hindbrain Present 5B , 5A
TS15: head mesenchyme Present Regionally restricted 5A , 5B Expression is present in the mesenchyme surrounding the anterior cardinal vein.
TS15: arterial system Present Regionally restricted 5A , 5B Expression is present in the endothelium.
Specimen 5P, 5Q: embryonic day 8.5; Efnb2tm1And/Efnb2+ (more )
Note: 12-13 somites. Double labeled: red - Efnb2 reporter; green - Pecam1 (assay MGI:2176794).
Structure Level Pattern Image Note
TS13: arterial system Present 5P , 5Q Authors report expression in vascular sprouts connecting the dorsal aorta and anterior cardinal vein.
TS13: dorsal aorta Present 5P , 5Q Authors report expression in vascular sprouts connecting the dorsal aorta and anterior cardinal vein.
TS13: head mesenchyme Present Regionally restricted 5Q , 5P Expression is present in the mesenchyme surrounding the dorsal aorta and the immature anterior cardinal vein plexus.
TS13: anterior cardinal vein Absent 5P , 5Q
Specimen 6A, 6B: embryonic day 9.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: red - Efnb2 reporter; green - Pecam1 (assay MGI:2176794).
Structure Level Pattern Image Note
TS15: internal carotid artery Present 6A , 6B
TS15: arterial system Present 6B , 6A Authors report expression in both endothelial and non-endothelial mesenchymal and neuroepithelial tissues.
TS15: future brain Present 6B , 6A Authors report expression in both endothelial and non-endothelial mesenchymal and neuroepithelial tissues.
TS15: head mesenchyme Present 6B , 6A Authors report expression in both endothelial and non-endothelial mesenchymal and neuroepithelial tissues.
Specimen 7A, 7B: embryonic day 8.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: red - Efnb2 reporter; green - Pecam1 (assay MGI:2176794).
Structure Level Pattern Image Note
TS13: dorsal aorta Present 7B , 7A
TS13: arterial system Present Regionally restricted 7A , 7B Expression in intersomitic sprouts from the dorsal aorta.
TS13: trunk somite Present Regionally restricted 7A , 7B Expression in the caudal half of each somite.
Specimen 8A, 8B: embryonic day 9.5; Efnb2tm1And/Efnb2+ (more )
Note: Double labeled: red - Efnb2 reporter; green - Pecam1 (assay MGI:2176794).
Structure Level Pattern Image Note
TS15: endocardial lining Present 8B , 8A
TS15: cardiac muscle tissue Weak 8B , 8A
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory