Antibody:	Anti-Aym1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: primary spermatocyte	Present		7B , 7A	Expression was localized mainly to primary spermatocytes at the periphery of the tubules.

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		7C , 7D

Structure	Level	Pattern	Image	Note
TS28: testis	Absent		7F , 7E

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		7H , 7G	Expression was detected in leptotene spermatocytes.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present	Regionally restricted	7I , 7J	Expression was detected mainly in the cells at the periphery of the tubules, spermatogonia cells and to a lesser degree to the more centrally located zygotene spermatocytes.
TS28: spermatocyte	Present		7J , 7I	Expression was detected in zygotene spermatocytes.
TS28: spermatogonium	Present		7I , 7J

Structure	Level	Pattern	Image	Note
TS28: testis	Weak		7K , 7L	Expression was significantly reduced and in some cases close to background level.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		7M , 7N	Most pachytene spermatocytes exhibited nuclear localization.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		7P , 7O	Expression was intense in nuclei of late meiotic (mainly diplotene) spermatocytes and possibly secondary spermatocytes.
TS28: spermatogonium	Present		7P , 7O

Structure	Level	Pattern	Image	Note
TS25: primary oocyte	Present		8A , 8B	Expression was detected in the nucleus of pachytene oocytes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		8D , 8C	An intense nuclear signal was detected in the diplotene meiotically arrested oocytes within undeveloped follicles.