Gene Expression Data
Assay Details
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Assay
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Reference:
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J:20325
Kachinsky AM, et al., Myogenesis and the intermediate filament protein, nestin. Dev Biol. 1994 Sep;165(1):216-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3790500
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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Rat-401 (MAB353)
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
not shown:
embryonic day 8.5
(more  )
Note: 6-7 somites.
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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6-7 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural crest |
Present |
Regionally restricted |
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Expression in a small number of cells on the dorsal surfaces of neural tube and somites, likely due to neural crest cells.
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| TS12: somite |
Present |
Regionally restricted |
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Expression among epithelial cells of rostral somites.
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Specimen
not shown:
embryonic day 8.5
(more )
Note: 9-10 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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9-10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: somite |
Present |
Regionally restricted |
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Expression in rostral somites in which myotomes had formed.
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Specimen
not shown:
embryonic day 9.0
(more )
Note: 15-16 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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15-16 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: myotome |
Present |
Regionally restricted |
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Expression in myotomal cells in the most rostral 6-7 somite pairs; no expression in remaining caudal somites
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Specimen
7A:
embryonic day 9.5
(more )
Note: 26 somites.
Specimen
7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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26 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
7A
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Filamentous pattern of expression appeared to outline all or most of cells of the dermatome; intense expression in the anterior lip of the dermatome.
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| TS15: trunk myotome |
Present |
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7A
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Specimen
7C:
embryonic day 10.5
(more )
Note: 35 somites.
Specimen
7C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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35 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Strong |
Regionally restricted |
7C
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Intense expression in a small number of dermatomal regions; outline pattern of dermatomal expression was less apparent.
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| TS17: trunk myotome |
Present |
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7C
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Specimen
7D:
embryonic day 9.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk myotome |
Present |
Regionally restricted |
7D
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Expression more intense at ends than in the middle of myotomal myocytes.
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Specimen
7E:
embryonic day 9.5
(more )
Specimen
7E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk myotome |
Present |
Spotted |
7E
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Expression in myotomal myoblasts; pattern of expression was discontinuous or dot-like.
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Specimen
7F:
embryonic day 9.5
(more )
Specimen
7F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk myotome |
Present |
Spotted |
7F
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Pattern of expression was discontinuous or dot-like.
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