Probe:	pint-1E5
Probe preparation:	Double stranded labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1.2

Structure	Level	Pattern	Image	Note
TS13: embryo ectoderm	Present	Regionally restricted	1.5	Expression in the region of the head folds, restricted to ectoderm overlying embryonic mesoderm.
TS13: head	Present	Regionally restricted	1.5	Expression in the region of the head folds, restricted to ectoderm overlying embryonic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1.8	In head folds, expression in all cells of the neural plate.
TS13: future spinal cord neural plate	Absent		1.8	No expression in the most posterior region of the neural plate.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1.11	In more caudal sections, expression progressively more restricted to lateral tips of neural plate.
TS13: future spinal cord neural plate	Absent		1.11	No expression in the most posterior region of the neural plate.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1.14	In more caudal sections, expression progressively more restricted to lateral tips of neural plate. `
TS13: future spinal cord neural plate	Absent		1.14	No expression in the most posterior region of the neural plate.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2.4	Expression in the dorsal wall of the diencephalon.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	2.6	No expression in more rostral parts of dorsal wall of forebrain.
TS17: midbrain	Present	Regionally restricted	2.6	Expression in dorsal wall of midbrain.

Structure	Level	Pattern	Image	Note
TS17: midbrain lateral wall	Present	Regionally restricted	2.8	Expression in lateral walls of midbrain close to junction with hindbrain.
TS17: diencephalon	Present	Regionally restricted	2.8	Expression in ventral walls of midbrain and diencephalon.
TS17: midbrain	Present	Regionally restricted	2.8	Expression in ventral walls of midbrain and diencephalon.

Structure	Level	Pattern	Image	Note
TS17: midbrain lateral wall	Present	Regionally restricted	2.10	Expression in lateral walls of midbrain close to junction with hindbrain.
TS17: diencephalon	Present	Regionally restricted	2.10	Expression in ventral walls of midbrain and diencephalon.
TS17: midbrain	Present	Regionally restricted	2.10	Expression in ventral walls of midbrain and diencephalon.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	2.12	No expression in rostral hindbrain.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	2.14	Expression in rhombic lips forming the junction between the thick pseudostratified epithelium and the thin roof of ependymal cells.

Structure	Level	Pattern	Image	Note
TS17: neural tube roof plate	Present		2.16	Expression in cells along the length of the rhombic lips, which become progressively closer in more caudal sections, merging to form a single cluster of dorsal cells constituting the roof plate of the spinal cord.

Structure	Level	Pattern	Image	Note
TS17: neural tube roof plate	Present			Expression in roof plate cells down entire length of spinal cord.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3.1	Dorsal expression in diencephalon, midbrain, rhombic lips and spinal cord.
TS17: future spinal cord	Present	Regionally restricted	3.1	Dorsal expression in diencephalon, midbrain, rhombic lips and spinal cord.
TS17: hindbrain	Present	Regionally restricted	3.1	Dorsal expression in diencephalon, midbrain, rhombic lips and spinal cord.
TS17: midbrain	Present	Regionally restricted	3.1	Dorsal expression in diencephalon, midbrain, rhombic lips and spinal cord.

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Present		3.2	Expression in lateral and ventral walls of midbrain.
TS17: midbrain lateral wall	Present		3.2	Expression in lateral and ventral walls of midbrain.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	3.3	Expression in dorsal walls of brain and spinal cord.
TS20: spinal cord	Present	Regionally restricted	3.3	Expression in dorsal walls of brain and spinal cord.
TS20: telencephalon	Absent		3.3	Rostral limit of expression is at the level of the crebral hemispheres.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	3.4	Expression in dorsal walls of brain and spinal cord.
TS20: spinal cord	Present	Regionally restricted	3.4	Expression in dorsal walls of brain and spinal cord.
TS20: midbrain floor plate	Present		3.4	Expression in the lateral and ventral walls of the midbrain.
TS20: midbrain lateral wall	Present		3.4	Expression in the lateral and ventral walls of the midbrain.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Absent		3.5	Rostral limit of expression in the dorsal wall of the brain is at the level of the cerebral hemispheres.

Structure	Level	Pattern	Image	Note
TS22: midbrain floor plate	Present		3.6	Expression detected in the lateral walls and caudal ventral wall of the midbrain.
TS22: midbrain lateral wall	Present		3.6	Expression detected in the lateral walls and caudal ventral wall of the midbrain.
TS22: diencephalon	Present	Regionally restricted	3.6	No expression in the ventral wall of the rostral midbrain or diencephalon.
TS22: midbrain	Present	Regionally restricted	3.6	No expression in the ventral wall of the rostral midbrain or diencephalon.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present	Regionally restricted	4.2	Expression in lateral and ventral walls of caudal midbrain, close to midbrain-hindbrain junction.
TS20: midbrain lateral wall	Present	Regionally restricted	4.2	Expression in lateral and ventral walls of caudal midbrain, close to midbrain-hindbrain junction.
TS20: diencephalon	Present	Regionally restricted	4.2	Additional expression in groups of cells in the ventral wall of the caudal diencephalon and in the rostral diencephalon, adjacent to the interventricular foramen.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4.4	Loop of expressing cells, linking strips of cells in the dorsal and ventral midbrain.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	4.6	Expression in the epithelium at the base of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	4.8	Expression in a group of cells in the dorsal neural tube, centered at the midline.