Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Pfkl)224Yg
Name:	transgene insertion 224, Yorum Groner
MGI ID:	MGI:3789607
Synonyms:	PFKL mini-gene
Transgene:	Tg(Pfkl)224Yg  Location: unknown
Alliance:	Tg(Pfkl)224Yg page

Strain of Origin:

(BALB/cBy x C57BL/6)F1 X (C57BL/6 x DBA/2)F1

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Pfkl)224Yg expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Pfkl (MGI:97547)   Mutation details: The transgenic construct for Tg(Pfkl)224Yg has 2.9 kb of the 5' genomic region, the first exon, first intron, and some of the second intron of Pfkl followed by the cDNA sequence extending to the 3' untranslated sequence and then genomic sequence from the 3' untranslated region was place downstream of this. A 22 base pair deletion was introduced into this 3' untranslated sequence in order to provide a system for detection of transgene-derived transcript. Line 224 was found to have several dozen copies of the transgene. (J:18425)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:18425 Elson A, et al., Overexpression of liver-type phosphofructokinase (PFKL) in transgenic-PFKL mice: implication for gene dosage in trisomy 21. Biochem J. 1994 Apr 15;299(Pt 2):409-15
All:	1 reference(s)