Gene Expression Data
Assay Details
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Assay
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Reference:
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J:132672
Wilson MD, et al., ARS2 is a conserved eukaryotic gene essential for early mammalian development. Mol Cell Biol. 2008 Mar;28(5):1503-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3785718
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Gene symbol:
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Srrt
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Gene name:
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serrate RNA effector molecule homolog (Arabidopsis)
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Antibody:
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XL12.2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
Ars2+/-:
embryonic day 3.5; Srrttm1Bfk/Srrt +
(more  )
Specimen
Ars2+/-:
(close  )
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Genetic Background:
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either: (involves: 129S1/Sv * 129S2/SvPasCrl * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * Crl:CD-1)
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Mutant Allele(s):
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Srrttm1Bfk/Srrt+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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6t=0 Ars2+/- Merge
,
6t=0 Ars2+/- anti-ARS2
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There is strong nuclear localization and diffuse cytoplasmic staining in all cells.
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Specimen
Ars2-/-:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129S2/SvPasCrl * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * Crl:CD-1)
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Mutant Allele(s):
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Srrttm1Bfk/Srrttm1Bfk
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Weak |
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6t=0 Ars2-/- anti-ARS2
,
6t=0 Ars2-/- Merge
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In mutant embryos expression is present in smaller amounts than in wild-type (or is even absent). In either case, there is a lack of nuclear localization.
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Analysis Tools