Probe:	stef cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1A	Expression is present in a portion of the telencephalon that includes the primorida of the cerebral cortex and hippocampus.
TS20: cerebral cortex	Present		1A

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		1B
TS25: corpus striatum	Present		1B
TS25: hippocampus	Present		1B
TS25: main olfactory bulb	Present		1B
TS25: brain subventricular zone	Present		1B	Expression is present in the rostral migratory pathway.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		1C

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1D

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1E

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1F
TS28: dentate gyrus	Strong		1F
TS28: main olfactory bulb	Strong		1F
TS28: brain subventricular zone	Strong		1F	There is strong expression in the rostral migratory pathway.
TS28: thalamus	Weak		1F	There is faint expression in a few thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	2A	Expression is present in the preplate, which consists of the Cajal-Retzius cells and the precursors of subplate neurons.
TS20: cortical subplate	Present		2A

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Present		2B	Expression is present in migrating cells.
TS25: cortical plate	Present		2B	Expression is present in neurons.
TS25: cerebral cortex ventricular zone	Absent		2B

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Strong		2C	There is strong expression in the cortical plate.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2D	Expression is present in the cortical plate.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2E	Expression in the cortical plate weakens during the postnatal period.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak		2F	Expression in the cortical plate weakens during the postnatal period.

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Strong		2G

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong		2H
TS27: dentate gyrus granule cell layer	Strong		2H

Structure	Level	Pattern	Image	Note
TS28: hippocampus region	Present	Non-Uniform	2I	Expression in the CA2 region is maintained in the adult stages, but expression in CA1 and CA3 regions decreases in the postnatal period.
TS28: dentate gyrus granule cell layer	Present		2I

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Non-Uniform	2J	Expression in the CA2 region is maintained in the adult stages, but expression in CA1 and CA3 regions decreases in the postnatal period.
TS28: dentate gyrus granule cell layer	Present		2J

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Non-Uniform	2K	Expression in the CA2 region is maintained in the adult stages, but expression in CA1 and CA3 regions decreases in the postnatal period.
TS28: dentate gyrus granule cell layer	Present		2K

Structure	Level	Pattern	Image	Note
TS28: hippocampus region	Present	Non-Uniform	2L	Expression in the CA2 region is maintained in the adult stages, but expression in CA1 and CA3 regions decreases in the postnatal period.
TS28: hippocampus CA2	Present		2L
TS28: dentate gyrus granule cell layer	Present		2L

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb	Present	Regionally restricted	2M	Expression is present in mitral cells.
TS25: brain subventricular zone	Present		2M	Expression is present in the rostral migratory pathway, where many cells are migrating from the subventcular zone of the lateral ventricle toward the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present	Regionally restricted	2N	Expression is present in the rostral migratory pathway.
TS28: main olfactory bulb granule cell layer	Present		2N
TS28: main olfactory bulb mitral cell layer	Present		2N
TS28: main olfactory bulb glomerular layer	Present		2N	Expression is present in periglomerular cells.
TS28: main olfactory bulb external plexiform layer	Absent		2N	There is no expression detected in tufted cells.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present	Regionally restricted	2O	Expression is present in the rostral migratory pathway.
TS28: main olfactory bulb granule cell layer	Present		2O
TS28: main olfactory bulb mitral cell layer	Present		2O
TS28: main olfactory bulb glomerular layer	Present		2O	Expression is present in periglomerular cells.
TS28: main olfactory bulb external plexiform layer	Absent		2O	There is no expression detected in tufted cells.

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present	Regionally restricted	2P	Expression is present in the rostral migratory pathway.
TS28: main olfactory bulb granule cell layer	Present		2P
TS28: main olfactory bulb mitral cell layer	Present		2P
TS28: main olfactory bulb glomerular layer	Present		2P	Expression is present in periglomerular cells.
TS28: main olfactory bulb external plexiform layer	Absent		2P	There is no expression detected in tufted cells.