Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114574
Salmon NA, et al., A gene trap knockout of the abundant sperm tail protein, outer dense fiber 2, results in preimplantation lethality. Genesis. 2006 Nov;44(11):515-22
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3783310
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Gene symbol:
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Odf2
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Gene name:
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outer dense fiber of sperm tails 2
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
3c:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Odf2Gt(RRO072)Byg/Odf2+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: seminiferous tubule |
Present |
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3c
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Expression is in round spermatids in the seminiferous tubule and in the region of the sperm tails within the lumen.
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| TS28: spermatid |
Present |
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3c
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Expression is in round spermatids in the seminiferous tubule and in the region of the sperm tails within the lumen.
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| TS28: spermatozoon |
Present |
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3c
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Expression is in round spermatids in the seminiferous tubule and in the region of the sperm tails within the lumen.
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Specimen
3d:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Odf2Gt(RRO072)Byg/Odf2+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatozoon |
Present |
Regionally restricted |
3d
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Expression is in a single spot within the cytoplasm and is not seen in the developing sperm tail.
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Specimen
3e:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Odf2Gt(RRO072)Byg/Odf2+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatozoon |
Present |
Regionally restricted |
3e
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In individual spermatozoa within the seminiferous tubule, expression is seen not in the tail portion itself, but within the cytoplasmic droplet attached to it.
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Specimen
3f:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Odf2Gt(RRO072)Byg/Odf2+
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epididymis |
Absent |
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3f
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No LacZ staining was seen within the caput epididymal sperm tails.
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Analysis Tools
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