Tg(tetO-Cxcl1,-lacZ)19Weik
Transgene Detail
|
|
| Symbol: |
Tg(tetO-Cxcl1,-lacZ)19Weik |
| Name: |
transgene insertion 19, Maria T Wiekowski |
| MGI ID: |
MGI:3779038 |
| Transgene: |
Tg(tetO-Cxcl1,-lacZ)19Weik Location: unknown
|
| Alliance: |
Tg(tetO-Cxcl1,-lacZ)19Weik page
|
|
| Strain of Origin: |
(C57BL/6J X DBA/2)F2
|
|
| Transgene Type: |
|
Transgenic (Inducible, Inserted expressed sequence, Reporter) |
| Inducer: |
|
doxycycline/tetracycline |
| Mutation: |
|
Insertion
|
| |
|
Mutation details: The construct encodes a bidirectional reporter transgene. The cDNA of the chemokine KC (Cxcl1, from +2 to +140) was cloned into the pBI-G vector (Clontech). The vector contains a tetracycline responsive element consisting of seven copies of the tet-operator (tetO) that is between 2 CMV minimal promoters which are silent in absence of an activator (tTA or rtTA) sequence. When activated, one promoter drives expression of Cxcl1 while the other promoter drives expression of a beta-galactosidase reporter gene.
(J:73096)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:73096 Wiekowski MT, et al., Disruption of neutrophil migration in a conditional transgenic model: evidence for CXCR2 desensitization in vivo. J Immunol. 2001 Dec 15;167(12):7102-10 |
| All: |
2 reference(s) |
|
Analysis Tools
