Probe:	mShisa3 cDNA1
Probe preparation:	labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Absent		4A

Structure	Level	Pattern	Image	Note
TS11: chorion	Absent		4B
TS11: yolk sac	Absent		4B

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		4C

Structure	Level	Pattern	Image	Note
TS16: foregut	Present		4D
TS16: hepatic primordium	Present		4D
TS16: nephric duct	Present		4D
TS16: somite	Present	Regionally restricted	4D	Expression was detected in the ventral portion of somites.
TS16: future hindbrain	Present	Regionally restricted	4D	Exclave-like expression was found in ventral mesencephalon and rhombencephalon.
TS16: future midbrain	Present	Regionally restricted	4D	Exclave-like expression was found in ventral mesencephalon and rhombencephalon.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted		Expression was detected in ventral midbrain and hindbrain except for isthmus.
TS17: midbrain	Present	Regionally restricted		Expression was detected in ventral midbrain and hindbrain except for isthmus.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	4E	Expression was detected in the ventral part of the midbrain.
TS20: metanephros	Present		4E
TS20: embryo mesenchyme	Present	Regionally restricted	4E	Expression was detected in myotome.

Structure	Level	Pattern	Image	Note
TS9: anterior visceral endoderm	Absent		7A

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Absent		7B	Expression was not detected in anterior mesendoderm.
TS11: neural ectoderm	Absent		7B	Expression was not detected in anterior neuroectoderm.