Probe:	mShisa probe1
Probe preparation:	labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS9: primitive endoderm	Present	Regionally restricted	1A	Expression was detected in the most anterior portion of the embryonic part of visceral endoderm.

Structure	Level	Pattern	Image	Note
TS9: primitive endoderm	Absent		1B	Expression in the visceral endoderm was absent.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	1C	Expression was detected in anterior mesendoderm.
TS11: neural ectoderm	Present	Regionally restricted	1C	Expression was detected in anterior neuroectoderm.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	1E , 1D	Expression was detected in anterior mesendoderm.
TS11: neural ectoderm	Present	Regionally restricted	1D , 1E	Expression was detected in anterior neuroectoderm.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Weak	Regionally restricted	1F	Expression in anterior mesendoderm was variable, but was greatly reduced in the majority of mutant embryos.
TS11: neural ectoderm	Weak	Regionally restricted	1F	Expression in anterior neuroectoderm was variable, but was greatly reduced in the majority of mutant embryos.

Structure	Level	Pattern	Image	Note
TS7: primitive endoderm	Weak	Regionally restricted		Expression was faintly found in distal visceral endoderm.

Structure	Level	Pattern	Image	Note
TS10: anterior visceral endoderm	Present		3A
TS10: embryo ectoderm	Present	Regionally restricted	3A	Expression was detected in the anterior ectoderm.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present		3B
TS12: future prosencephalon	Present		3B
TS12: somite	Present		3B
TS12: trunk unsegmented mesenchyme	Weak		3B

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present		3C
TS12: future prosencephalon	Present		3C

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present		3D
TS15: somite	Present		3D
TS15: surface ectoderm	Present	Regionally restricted	3D	Expression was detected in surface ectoderm of anterior forebrain region.
TS15: head mesenchyme	Present		3D
TS15: future hindbrain	Present	Regionally restricted	3D	Expression was detected in ventral hindbrain.
TS15: otic pit	Present	Regionally restricted	3D	Expression was detected in otic cup.
TS15: mesonephric tubule	Weak		3D
TS15: branchial pouch	Absent		3D

Structure	Level	Pattern	Image	Note
TS14: branchial arch mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3E
TS20: inner ear	Present	Regionally restricted	3E	Expression was detected in the ventral part of the otic placode including adjacent mesenchyme.
TS20: tongue	Present	Regionally restricted	3E
TS20: hypothalamus	Present	Regionally restricted	3E	Expression was deteced in the preoptic area neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3F

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3G
TS20: hypothalamus	Present		3G
TS20: lateral ganglionic eminence	Present		3G
TS20: inner ear	Present	Regionally restricted	3G	Expression was detected in the ventral part of the otic placode including adjacent mesenchyme.
TS20: embryo mesenchyme	Present	Regionally restricted	3G	Expression was detected in myotome.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Present	Regionally restricted	3H	Expression was detected in endocardial cushion around aortic valve.
TS20: embryo mesenchyme	Present	Regionally restricted	3H	Expression was detected in mesenchymal tissues including muscle primordia.