Cryaatm1.1Ady
Targeted Allele Detail
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| Symbol: |
Cryaatm1.1Ady |
| Name: |
crystallin, alpha A; targeted mutation 1.1, Usha P Andley |
| MGI ID: |
MGI:3777403 |
| Synonyms: |
alpha-R49C knock-in |
| Gene: |
Cryaa Location: Chr17:31896905-31900704 bp, + strand Genetic Position: Chr17, 17.09 cM, cytoband A3-B
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| Alliance: |
Cryaatm1.1Ady page
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Lens opacity in Cryaatm1.1Ady/Cryaa+, and Cryaatm1.1Ady/Cryaatm1.1Ady mice and microphthalmia in Cryaatm1.1Ady/Cryaatm1.1Ady mice
Show the 4 phenotype image(s) involving this allele.
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| Germline Transmission: |
Earliest citation of germline transmission:
J:132296
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129
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| Allele Type: |
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Targeted |
| Mutation: |
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Nucleotide substitutions
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Mutation details: The codon for arginine 49 was altered to cysteine by homologous recombination. A loxP flanked neomycin cassette was introduced between exon 1 and 2, then subsequently was deleted by crossing with cre expressing transgenic mice. Mass spectrometry analyses confirmed alpha-R49C mutant protein expression in the mutant lenses.
(J:132296)
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Generation of the Cryaatm1Ady anad Cryaatm1.1Ady alleles |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cryaa Mutation: |
14 strains or lines available
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| Original: |
J:132296 Xi JH, et al., Mechanism of small heat shock protein function in vivo: a knock-in mouse model demonstrates that the R49C mutation in alpha A-crystallin enhances protein insolubility and cell death. J Biol Chem. 2008 Feb 29;283(9):5801-14 |
| All: |
10 reference(s) |
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Analysis Tools
