Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:98502
Strumpf D, et al., Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development. 2005 May;132(9):2093-102
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3777396
|
|
Gene symbol:
|
Tjp1
|
|
Gene name:
|
tight junction protein 1
|
|
|
Results
|
Specimen
3A:
embryonic day 3.25; Cdx2tm1Fbe/Cdx2 +
(more  )
Note: This is an early blastocyst. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:3777394).
Specimen
3A:
(close  )
|
Genetic Background:
|
involves: ICR
|
|
Mutant Allele(s):
|
Cdx2tm1Fbe/Cdx2+
|
|
Age:
|
embryonic day 3.25
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
This is an early blastocyst. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:3777394).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Regionally restricted |
3A
|
This protein localizes to the tight junctions.
|
Specimen
3B:
embryonic day 3.25; Cdx2tm1Fbe/ Cdx2tm1Fbe
(more )
Note: This is an early blastocyst. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:3777394).
Specimen
3B:
(close )
|
Genetic Background:
|
involves: ICR
|
|
Mutant Allele(s):
|
Cdx2tm1Fbe/Cdx2tm1Fbe
|
|
Age:
|
embryonic day 3.25
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
This is an early blastocyst. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:3777394).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
Regionally restricted |
3B
|
This protein localizes to the tight junctions. Its localization appears grossly normal in mutants compared to non-mutant genotypes.
|
Specimen
3E:
embryonic day 4.0; Cdx2tm1Fbe/Cdx2 +
(more )
Note: This is a late blastocyst.
Specimen
3E:
(close )
|
Genetic Background:
|
involves: ICR
|
|
Mutant Allele(s):
|
Cdx2tm1Fbe/Cdx2+
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
This is a late blastocyst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: trophectoderm |
Present |
Regionally restricted |
3E
|
This protein localizes to the tight junctions.
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: ICR
|
|
Mutant Allele(s):
|
Cdx2tm1Fbe/Cdx2tm1Fbe
|
|
Age:
|
embryonic day 4.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Note:
|
This is a late blastocyst.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: trophectoderm |
Present |
Regionally restricted |
3F
|
This protein localizes to the tight junctions. In mutants the staining appears patchy and diffuse, suggesting the integrity of the epithelium is not maintained in the mutants.
|
|
Analysis Tools