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Gene Expression Data
Assay Details
Assay
Reference: J:130330 Ralston A, et al., Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol. 2008 Jan 15;313(2):614-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777238
Gene symbol: Krt8
Gene name: keratin 8
Antibody: TROMA 1
Detection system: Secondary antibody
Results
Specimen 4A: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 4A In the majority of embryos at this stage expression is unlocalized within the cells.
Specimen 4B: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Non-Uniform 4B In a minority of embryos at this stage expression is apically localized.
Specimen 4C: embryonic day 3.0 (more )
Note: 20-cell embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 4C Expression is present in all cells; it is detected at the entire cell membrane.
Specimen 4D: embryonic day 3.5 (more )
Note: 33-cell embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present Non-Uniform 4D Expression levels are reduced in inside cells.
Specimen 4F: embryonic day 3.0 (more )
Note: 14-cell embryo. Double labeled: green - Krt8; red - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: compacted morula Present 4F Krt8
Specimen 4G: embryonic day 3.5 (more )
Note: 33-cell embryo. Double labeled: green - Krt8; red - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: blastocyst Present 4G Krt8
Specimen 5A: embryonic day 3.5 (more )
Note: This is a blastocyst stage embryo. Double labeled: red - Krt8; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: trophectoderm Present Non-Uniform 5A Krt8 Expression is enriched in the cell membrane.
Specimen 5B: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst stage embryo. Double labeled: red - Krt8; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: trophectoderm Present 5B Krt8 Expression levels are reduced compared to those seen in wild type, but it is still localized to the membrane.
Specimen 5C: embryonic day 3.0 (more )
Note: 16-cell embryo. Double labeled: red - Krt8; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 5C Krt8 Apical expression is present.
Specimen 5D: embryonic day 3.0; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: 16-cell embryo. Double labeled: red - Krt8; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 5D Krt8 Expression levels are reduced compared to those seen in wild type, but it is still apically localized.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory